# US 8,785,202 B2 · Colloidal metal compositions and methods

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- Number: US 8,785,202 B2
- Document ID: US8785202B2
- Status: Granted
- Kind: B2
- Application number: 12/708,326
- Filed: 2010-02-18
- Published: 2010-06-10
- Granted: 2014-07-22
- Earliest claimed: 2001-04-30
- Assignees: CytImmune Sciences, Inc.
- Applicants: Lawrence Tamarkin; Giulio F. Paciotti
- Inventors: Lawrence Tamarkin; Giulio F. Paciotti
- Web page: https://patentgrep.com/patent/US8785202B2

## CPC

- C07K 14/525: Tumour necrosis factor [TNF]
- A61K 38/191: {Tumor necrosis factors [TNF], e.g. lymphotoxin [LT], i.e. TNF-beta}
- A61K 38/39: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
- A61K 39/39: characterised by the immunostimulating additives, e.g. chemical adjuvants
- A61K 47/60: the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
- A61K 47/6923: {the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb}
- A61K 49/0013: {Luminescence}
- A61K 49/0017: {Fluorescence in vivo}
- A61K 49/0056: {Peptides, proteins, polyamino acids}
- A61K 49/0065: {the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle}
- A61K 51/1244: {microparticles or nanoparticles, e.g. polymeric nanoparticles}
- A61P 35/00: Antineoplastic agents
- A61P 35/04: specific for metastasis
- A61P 37/04: Immunostimulants
- C07K 16/241: {Tumor Necrosis Factors}
- A61K 2039/55505: {Inorganic adjuvants}
- A61K 2039/55516: {Proteins; Peptides}
- A61K 2039/55522: {Cytokines; Lymphokines; Interferons}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention comprises compositions and methods for delivery systems of agents, including therapeutic compounds, pharmaceutical agents, drugs, detection agents, nucleic acid sequences and biological factors. In general, these vector compositions comprise a colloidal metal, derivatized PEG (polyethylene glycol) and an agent. The invention also comprises methods and compositions for making such colloidal metal compositions and for treatment of cancer.

## Claims

### Claim 1

A method for detecting a solid tumor, comprising;
site-directed accumulation of a detection agent in a tumor achieved by administering to an organism having a solid tumor, a vector composition consisting of a pegylated colloidal gold metal particle, and at least one detection agent, wherein a polyethylene glycol (PEG) thiol derivative is covalently bound to the colloidal gold metal, wherein the detection agent is a radioactive detection agent, a fluoresescent detection agent, a chemiluminescent detection agent, or a magnetic detection agent, and
detecting the composition, wherein detection of the composition indicates the presence of a solid tumor.

### Claim 2 (depends on 1)

The method of claim 1, wherein the detection agent is a radioactive detection agent.

### Claim 3 (depends on 1)

The method of claim 1, wherein the detection agent is a magnetic detection agent.

### Claim 4 (depends on 1)

The method of claim 1, wherein the detection agent is a fluorescent detection agent.

### Claim 5 (depends on 1)

The method of claim 1, wherein the detection agent is a chemiluminescent detection agent.

### Claim 6 (depends on 1)

method of claim 1, wherein the detection agent is bound to the colloidal gold metal.

### Claim 7 (depends on 6)

The method of claim 6, wherein the detection agent is bound to the colloidal gold metal by an integrating molecule.

### Claim 8 (depends on 7)

The method of claim 7, wherein the integrating molecule is a polycationic molecule.

### Claim 9 (depends on 8)

The method of claim 8, wherein the polycationic molecule is selected from the group consisting of; polylysine, histones, protamine sulfate, and asioalogylcoproteins.

### Claim 10 (depends on 7)

The method of claim 7, wherein the integrating molecule is a binding pair.

### Claim 11 (depends on 10)

The method of claim 10, wherein the binding pair is selected from the group consisting of; antibody-antigen pairs, enzyme substrate pairs, receptor-ligand pairs, and streptavidin-biotin.

### Claim 12 (depends on 11)

The method of claim 11, wherein the binding pair is streptavidin-biotin.

### Claim 13 (depends on 1)

The method of claim 1, wherein the PEG derivative is a heterofunctional PEG with the general structure X-PEG-Y, wherein X is the detection agent and Y is a thiol functional group.