# US 8,785,191 B2 · Concentration of stem cells obtained during orthopaedic surgeries

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- Number: US 8,785,191 B2
- Document ID: US8785191B2
- Status: Granted
- Kind: B2
- Application number: 12/199,743
- Filed: 2008-08-27
- Published: 2009-03-05
- Granted: 2014-07-22
- Earliest claimed: 2007-08-27
- Assignees: University of Connecticut
- Applicants: Augustus D. Mazzocca; Mary Beth McCarthy
- Inventors: Augustus D. Mazzocca; Mary Beth McCarthy
- Web page: https://patentgrep.com/patent/US8785191B2

## CPC

- C12N 5/0663: {Bone marrow mesenchymal stem cells (BM-MSC)}
- A61P 19/00: Drugs for skeletal disorders
- A61K 2035/124: {the cells being hematopoietic, bone marrow derived or blood cells}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Methods for isolating and concentrating bone marrow stromal cells drawn from various surgical sites (for example, the proximal humeral head during rotator cuff repair, or the distal femur during ACL surgery) during arthroscopic or open orthopaedic surgery. The pluripotent cells obtained from the bone marrow aspirate can then be reimplanted during the same surgery to improve healing.

## Claims

### Claim 1

A method of conducting arthroscopic surgery, the method consisting of the steps of:
harvesting a bone marrow aspirate from proximal humeral head or from distal femur undergoing arthroscopic or open orthopedic surgery;
subjecting, during the arthroscopic or open orthopedic surgery, the bone marrow aspirate to a centrifugation process for 5 minutes to obtain concentrated multipotent cells of the bone marrow aspirate and a residual component;
separating the concentrated multipotent cells from the residual component; and
re-implanting, during the same arthroscopic or open orthopedic surgery, the concentrated and separated multipotent cells into the proximal humeral head or the distal femur, or to the proximal humeral head or the distal femur, to promote tissue growth.

### Claim 2 (depends on 1)

The method of claim 1, wherein the arthroscopic or open orthopedic surgery is rotator cuff repair or ACL repair.