# US 8,785,189 B2 · Method of forming dendritic cells from embryonic stem cells

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- Number: US 8,785,189 B2
- Document ID: US8785189B2
- Status: Granted
- Kind: B2
- Application number: 13/859,228
- Filed: 2013-04-09
- Published: 2013-09-05
- Granted: 2014-07-22
- Earliest claimed: 2005-06-01
- Assignees: Wisconsin Alumni Research Foundation
- Applicants: Wisconsin Alumni Research Foundation
- Inventors: Igor I. Slukvin; James A. Thomson; Maksym A. Vodyanyk; Maryna E. Gumenyuk
- Web page: https://patentgrep.com/patent/US8785189B2

## CPC

- C12N 5/0639: {Dendritic cells, e.g. Langherhans cells in the epidermis}
- A61K 40/19: Dendritic cells
- A61K 40/24: Antigen-presenting cells [APC]
- A61K 40/46: Viral antigens
- A61P 35/00: Antineoplastic agents
- A61P 37/04: Immunostimulants
- C12N 5/064: {Immunosuppressive dendritic cells}
- C12N 2506/02: from embryonic cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

## Claims

### Claim 1

A culture of human regulatory dendritic cells in which majority of the cells express CD1alow, CD80low, CD86low, CD9− phenotype, display diminished ability to induce the proliferation of naïve T cells, and have tolerogenic properties.