# US 8,785,179 B2 · Biosensor and method

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- Number: US 8,785,179 B2
- Document ID: US8785179B2
- Status: Granted
- Kind: B2
- Application number: 10/152,745
- Filed: 2002-05-22
- Published: 2003-11-27
- Granted: 2014-07-22
- Earliest claimed: 2002-05-22
- Assignees: Texas Instruments Incorporated
- Applicants: John G. Quinn; Jerry Elkind
- Inventors: John G. Quinn; Jerry Elkind
- Web page: https://patentgrep.com/patent/US8785179B2

## CPC

- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- B05D 1/185: {applying monomolecular layers (B05D1/204 takes precedence)}
- C07C 321/02: Thiols having mercapto groups bound to acyclic carbon atoms
- C07C 391/02: having selenium atoms bound to carbon atoms of six-membered aromatic rings
- G01N 33/553: Metal or metal coated
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B05D: PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL (conveying articles or workpieces through baths of liquid B65G, e.g. B65G49/02)
- C07C: ACYCLIC OR CARBOCYCLIC COMPOUNDS

## Abstract

Surface plasmon resonance (SPR) sensor biointerface with a rigid thiol linker layer and/or interaction layer ligand loading with reversible collapse and/or iron oxide nanoparticle sensor response amplification.

## Claims

### Claim 1

A surface Plasmon resonance sensor structure for sensing a target analyte, comprising:
a metal sensor film having a metal surface,
a biointerface comprising a monolayer bound to said metal surface, said monolayer formed from a plurality of units, said plurality of units including units with the structure XR—Y wherein:
(i) X is selected from the group consisting of —S—, —S—S—, —Se—, —Se—Se—, —S—Se—, and mixtures thereof where “—” indicates a chemical bond;
(ii) R is a carbon backbone of chain length of at least 6 carbon atoms including double or triple bonds along said chain length; and
(iii) Y contains a functional group at a surface of said monolayer,
wherein said target analyte is bound to said functional group, and
a plurality of amorphous iron oxide nanoparticles having at least one ligand attached thereto that has structure for binding to said target analyte, wherein said ligand is bound to said target analyte,
wherein said functional group of said Y is selected from a group consisting of tris-hydroxymethyl ethyl groups, gem-ethyl diol, fluoro gem-propyl diol, and ethylamide.

### Claim 2 (depends on 1)

The structure of claim 1, wherein:
(a) said R is selected from the group consisting of aromatic rings, aromatic rings paraconnected by linear alkynes, and direct paraconnected aromatic rings.

### Claim 3 (depends on 2)

The structure of claim 2, wherein the direct para-connected aromatic rings are biphenyl.

### Claim 4 (depends on 1)

The sensor structure of claim 1, further comprising a hydrogel coating covalently attached to said plurality of amorphous iron oxide nanoparticles and being between said plurality of amorphous iron oxide nanoparticles and said ligand.

### Claim 5 (depends on 4)

The sensor structure of claim 4, wherein said hydrogel coating comprises a carboxymethylated dextran layer.