# US 8,785,172 B2 · Savinase variants having an improved wash performance on egg stains

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- Number: US 8,785,172 B2
- Document ID: US8785172B2
- Status: Granted
- Kind: B2
- Application number: 13/555,504
- Filed: 2012-07-23
- Published: 2012-11-22
- Granted: 2014-07-22
- Earliest claimed: 2006-04-20
- Assignees: Novozymes A/S
- Applicants: Stefan Minning; Jurgen Carsten Franz Knotzel; Niels Henrik Sorensen
- Inventors: Stefan Minning; Jurgen Carsten Franz Knotzel; Niels Henrik Sorensen
- Web page: https://patentgrep.com/patent/US8785172B2

## CPC

- C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase}
- C11D 3/38627: {containing lipase}
- C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase}
- C11D 3/38645: {containing cellulase}
- C11D 3/38654: {containing oxidase or reductase}
- C12N 9/54: bacteria being Bacillus
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Subtilase variants having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.

## Claims

### Claim 1

An isolated subtilase variant comprising either
(a) the amino acid sequence of SEQ ID NO:12 modified by the amino acid substitutions S3T, V4I, S99D, S101R, S103A, V104I, G160S, V205I, and L217D, or
(b) the amino acid sequence of SEQ ID NO:12 modified by the amino acid substitutions S3T, V4I, S99D, S101R, S103A, V104I, G160S, A194P, V205I, and L217D,
wherein each amino acid position is numbered by correspondence to a position in the amino acid sequence of subtilisin BPN′ as set forth in SEQ ID NO:11.

### Claim 2 (depends on 1)

The subtilase variant of claim 1, further comprising at least one further modification in one of the positions 27, 36, 56, 76, 87, 95, 96, 97, 98, 100, 102, 120, 123, 159, 167, 170, 206, 218, 222, 224, 232, 235, 236, 245, 248, 252 and 274, wherein each amino acid position is numbered by correspondence to a position in the amino acid sequence of subtilisin BPN′ as set forth in SEQ ID NO:11.

### Claim 3 (depends on 2)

The subtilase variant of claim 2 wherein the modification is selected from the group consisting of K27R, *36D, S56P, N76D, S87N, G97N, H120D, N123S, G159D, Q206E, N218S, M222S, M222A, T224S, A232V, K235L, Q236H, Q245R, N248D, N252K and T274A.

### Claim 4 (depends on 1)

A cleaning or detergent composition comprising the subtilase variant of claim 1.

### Claim 5 (depends on 4)

The composition of claim 4, which additionally comprises a cellulase, lipase, cutinase, oxidoreductase, another protease, an amylase, a pectate lyase, a mannanase or a mixture thereof.

### Claim 6 (depends on 4)

A method for cleaning or dish washing, washing a hard surface or laundry, the method comprising contacting the hard surface or the laundry with the composition of claim 4.

### Claim 7 (depends on 4)

A method for removal of egg stains from a hard surface or from laundry, the method comprising contacting the egg stain-containing hard surface or the egg stain-containing laundry with the composition of claim 4.