# US 8,785,171 B2 · Fabric and home care products comprising cold water proteases

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- Number: US 8,785,171 B2
- Document ID: US8785171B2
- Status: Granted
- Kind: B2
- Application number: 12/963,681
- Filed: 2010-12-09
- Published: 2011-09-29
- Granted: 2014-07-22
- Earliest claimed: 2009-12-09
- Assignees: The Procter & Gamble Company
- Applicants: Philip Frank Souter; Glenn Steven Ward; Viktor Yuryevich Alekseyev; Joshua Roy Basler; Luis Gustavo Cascão-Pereira; David A. Estell; Ayrookaran J. Poulose; James T. Kellis, Jr.; Alexander Pisarchik; Daniel Esteban Torres-Pazmino
- Inventors: Philip Frank Souter; Glenn Steven Ward; Viktor Yuryevich Alekseyev; Joshua Roy Basler; Luis Gustavo Cascão-Pereira; David A. Estell; Ayrookaran J. Poulose; James T. Kellis, Jr.; Alexander Pisarchik; Daniel Esteban Torres-Pazmino
- Web page: https://patentgrep.com/patent/US8785171B2

## CPC

- C11D 3/38618: {Protease or amylase in liquid compositions only}
- C11D 3/38609: {Protease or amylase in solid compositions only}
- C11D 3/38681: {Chemically modified or immobilised enzymes}
- C12N 9/50: Proteinases {, e.g. Endopeptidases (3.4.21-3.4.25)}
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN′ subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.

## Claims

### Claim 1

A composition comprising an isolated protein comprising a protease variant wherein
(i) the protease variant has at least 95% identity to SEQ ID NO: 6,
(ii) the protease variant comprises, compared to the parent protease of SEQ ID NO: 2, the substitutions S024G+S053G+S078N+S101N+G128A+Y217Q, and
(iii) the protease variant has suc-AAPF-pNA proteolytic activity.

### Claim 2 (depends on 1)

The composition according to claim 1, wherein said protease variant has a total net charge of −1, 0 or +1 relative to the parent protease of SEQ ID NO: 2.

### Claim 3 (depends on 1)

The composition of claim 1, said composition comprising an adjunct ingredient selected from the group consisting of: a surfactant, a builder, a chelating agent, a dye transfer inhibiting agent, a dispersant, one or more additional enzymes, an enzyme stabilizer, a catalytic material, a bleach activator, a hydrogen peroxide, a source of hydrogen peroxide, a preformed peracid, a polymeric dispersing agent, a clay soil removal/anti-redeposition agent, a brightener, a suds suppressor, a dye, a perfume, a perfume delivery system, a structure elasticizing agent, a fabric softener, a carrier, a hydrotrope, a processing aid, a solvent, a pigment and mixtures thereof.

### Claim 4 (depends on 1)

The composition according to claim 1, said composition comprising a second non-immunoequivalent protease that is a subtilisin (EC 3.4.21.62) protease.

### Claim 5 (depends on 1)

The composition of claim 1, said composition comprising an additional enzyme selected from the group consisting of hemicellulases, peroxidases, proteases, cellulases, cellobiose dehydrogenases, xyloglucanases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, lichenases glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, amylases, and mixtures thereof.

### Claim 6 (depends on 1)

The composition of claim 1, wherein said composition is a detergent composition.