# US 8,785,154 B2 · Method for manufacturing an aqueous glucose solution from plants of the Triticeae species > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,785,154 B2 - Document ID: US8785154B2 - Status: Granted - Kind: B2 - Application number: 12/937,554 - Filed: 2009-04-09 - Published: 2011-02-10 - Granted: 2014-07-22 - Earliest claimed: 2008-04-14 - Assignees: BASF SE - Applicants: Matthias Boy; Stephan Freyer; Julia Brodersen - Inventors: Matthias Boy; Stephan Freyer; Julia Brodersen - Web page: https://patentgrep.com/patent/US8785154B2 ## CPC - C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60) - A23K 10/12: by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass - A23K 20/147: Polymeric derivatives, e.g. peptides or proteins - C12P 21/00: Preparation of peptides or proteins (single cell protein C12N1/00) - C13K 1/06: obtained by saccharification of starch or raw materials containing starch - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - A23K: FODDER - C13K: SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES (chemically synthesised sugars or sugar derivatives C07H; polysaccharides, e.g. starch, derivatives thereof C08B; malt C12C; fermentation or enzyme-using processes for preparing compounds containing saccharide radicals C12P19/00; production of sucrose C13B) ## Abstract The present invention relates to a method for manufacturing an aqueous glucose solution from the starch components of Triticeae grains, for example from rye, triticale or in particular wheat grains. The invention also relates to a glucose-based fermentation method for manufacturing organic compounds in which the glucose manufactured for fermentation is produced from the starch components of Triticeae grains by way of a method according to the invention. ## Claims ### Claim 1 A method for producing an aqueous glucose solution having a glucose content of at least 32% by weight from starch of wheat grains of Triticeae plants, comprising: a) fractionating dry milling of wheat grains of Triticeae plants to obtain a milled material, wherein the grains are separated into a starch-comprising endosperm fraction and a bran fraction, and wherein said milled material comprises essentially the starch-comprising endosperm fraction; b) converting the endosperm fraction milled material into an aqueous suspension, wherein the aqueous suspension comprises 30 to 55% by weight of starch based on total weight of the aqueous suspension; and c) enzymatically liquefying and enzymatically saccharifying the starch of the aqueous suspension to obtain an aqueous glucose solution, wherein the aqueous glucose solution has a glucose content of at least 32% by weight; and wherein gluten present in the endosperm fraction is depleted from the aqueous glucose solution obtained in step c) and/or from the aqueous suspension of the endosperm fraction before carrying out step c). ### Claim 2 (depends on 1) The method according to claim 1, wherein the milling in step a) is carried out in the presence of 10 to 30% by weight of water based on the mass of the grains used. ### Claim 3 (depends on 1) The method according to claim 1, wherein, in step a), the endosperm fraction is milled to a median particle size in the range from 0.01 to 1.0 mm. ### Claim 4 (depends on 1) The method according to claim 1, wherein at least 70% of the gluten is separated off, based on total gluten present in the endosperm fraction used. ### Claim 5 (depends on 1) The method according to claim 1, wherein at least some of the gluten of the endosperm fraction is depleted from the aqueous glucose solution obtained in step c). ### Claim 6 (depends on 1) The method according to claim 1, wherein some of the gluten of the endosperm fraction is depleted before carrying out step c). ### Claim 7 (depends on 6) The method according to claim 6, wherein depleting the gluten before carrying out step c) comprises the following substeps: i) converting some of the endosperm fraction into a dilute aqueous suspension of the endosperm fraction having a starch content of less than 30% by weight; ii) depleting the gluten from the dilute aqueous suspension of the endosperm fraction to obtain a gluten-depleted dilute aqueous suspension of the endosperm fraction; and iii) suspending the remainder of the endosperm fraction in the gluten-depleted dilute aqueous suspension obtained in step ii) to obtain an aqueous suspension comprising a starch content of at least 30% to 50% by weight. ### Claim 8 (depends on 6) The method according to claim 6, wherein the remainder of the gluten of the endosperm fraction is depleted from the aqueous glucose solution obtained in step c). ### Claim 9 (depends on 1) The method according to claim 1, wherein the gluten is depleted in such a manner that the aqueous glucose solution comprises less than 10% by volume of solids. ### Claim 10 (depends on 1) A method for producing an organic substance by fermentation, which comprises the following steps: i. providing a glucose solution by a method according to claim 1 and ii. adding the glucose solution to a fermentation medium which comprises a microorganism which is capable of overproduction of the organic substance. ### Claim 11 (depends on 10) The method according to claim 10, wherein the organic substance is selected from optionally hydroxyl-bearing mono-, di- and tricarboxylic acids having 3 to 10 carbon atoms, proteinogenic and non-proteinogenic amino acids, purine bases, pyrimidine bases; nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols having 4 to 10 carbon atoms, polyhydric alcohols having 3 or more hydroxyl groups, long-chain alcohols having at least 4 carbon atoms, carbohydrates, aromatic compounds, vitamins, provitamins, cofactors, nutraceuticals, proteins, yeasts, carotenoids, ketones having 3 to 10 carbon atoms, lactones, polyhydroxyalkanoates, polylactides, polysaccharides, polyisoprenoids, polyamides and cyclodextrins. ### Claim 12 (depends on 11) The method according to claim 11, wherein the organic substance is an amino acid. ### Claim 13 (depends on 12) The method according to claim 12, wherein the amino acid is selected from lysine, methionine, threonine and glutamate. ### Claim 14 (depends on 11) The method according to claim 11, wherein the organic substance is selected from vitamins and provitamins. ### Claim 15 (depends on 11) The method according to claim 11, wherein the organic substance is selected from aliphatic mono-, di- and tricarboxylic acids having 2 to 10 carbon atoms. ### Claim 16 (depends on 11) The method according to claim 11, wherein the organic substance is selected from aliphatic hydroxycarboxylic acids having 3 to 10 carbon atoms. ### Claim 17 (depends on 11) The method according to claim 11, wherein the organic substance is selected from alkanediols having 3 to 10 carbon atoms. ### Claim 18 (depends on 11) The method according to claim 11, wherein the organic substance is selected from aliphatic ketones having 3 to 10 carbon atoms. ### Claim 19 (depends on 11) The method according to claim 11, wherein the organic substance is selected from aliphatic diamines having 3 to 10 carbon atoms. ### Claim 20 (depends on 11) The method according to claim 11, wherein the organic substance is selected from nucleotides. ### Claim 21 (depends on 11) The method according to claim 11, wherein the organic substance is selected from disaccharides, oligosaccharides and polysaccharides. ### Claim 22 (depends on 10) The method according to claim 10, wherein the biomass resulting from the microorganism is separated off from the overproduced organic substance of the fermentation product, and wherein a biomass-comprising composition is obtained. ### Claim 23 (depends on 1) A method for producing an organic substance by a reaction not involving fermentation, comprising i) providing an aqueous solution according to claim 1; and ii) using the thus obtained glucose solution or an essentially water-free glucose obtained by concentrating said glucose solution to a water content of less than 10% by weight in a reaction not involving fermentation for producing the desired organic substance.