# US 8,785,144 B2 · Electric analysis method

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- Number: US 8,785,144 B2
- Document ID: US8785144B2
- Status: Granted
- Kind: B2
- Application number: 12/922,971
- Filed: 2009-03-17
- Published: 2011-01-20
- Granted: 2014-07-22
- Earliest claimed: 2008-03-17
- Assignees: Mitsubishi Chemical Medience Corporation
- Applicants: Yasuo Ifuku; Nagamoto Murai
- Inventors: Yasuo Ifuku; Nagamoto Murai
- Web page: https://patentgrep.com/patent/US8785144B2

## CPC

- G01N 33/5438: {Electrodes}
- G01N 33/581: {with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)}
- G01N 2333/02: Hepadnaviridae, e.g. hepatitis B virus
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Disclosed is an analysis method comprising the steps of: (a) reacting a substance to be analyzed with at least a specific partner which exhibits a selective interaction with the substance, converting a soluble substance to an insoluble substance by an insolubilization reaction, in correlation with the amount of the substance to be analyzed contained in a sample, and depositing the insoluble substance on a sensing part, and (b) electrically analyzing the insoluble substance deposited on the sensing part, wherein at least one of steps (a) and (b) is carried out under flow conditions.

## Claims

### Claim 1

An analysis method characterized by comprising the steps of:
(a) reacting a substance to be analyzed with at least a specific partner which exhibits a selective interaction with the substance, converting a soluble substance to an insoluble substance by an insolubilization reaction, in correlation with the amount of the substance to be analyzed contained in a sample, and depositing the insoluble substance on a sensing part, and
(b) electrically analyzing the insoluble substance deposited on the sensing part, wherein the deposition of the insoluble substance in step (a) is carried out under flow conditions.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the specific partner is an enzyme.

### Claim 3 (depends on 1)

The method according to claim 1, wherein step (a) comprises:
(1) forming a complex comprising a substance to be analyzed, a specific partner which exhibits a selective interaction with the substance, and a labeling substance, in correlation with the amount of the substance to be analyzed contained in a sample, and
(2) converting a soluble substance to an insoluble substance by an insolubilization reaction directly or indirectly caused by the labeling substance contained in the formed complex, and depositing the insoluble substance on a sensing part, wherein the deposition of the insoluble substance in step (2) is carried out under flow conditions.

### Claim 4 (depends on 3)

The method according to claim 3, wherein the labeling substance is a hydrolase.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the hydrolase is alkaline phosphatase.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the insolubilization reaction is an oxidation-reduction reaction.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the soluble substance is selected from an inorganic ion, an organic ion, an enzyme substrate or its reaction product, and a dye.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the soluble substance is a metal ion.

### Claim 9 (depends on 8)

The method according to claim 8, wherein the metal ion is a silver ion.

### Claim 10 (depends on 1)

The method according to claim 1, wherein the sensing part is composed of any one of a metal, a polymer, carbon, a nanotube structure, graphite, or inorganic substance, or a combination thereof.

### Claim 11 (depends on 1)

The method according to claim 1, wherein the sensing part has one or more three-dimensional structures having an acute-angle-like shape.

### Claim 12 (depends on 1)

The method according to claim 1, wherein the specific partner is immobilized on the sensing part.

### Claim 13 (depends on 1)

The method according to claim 1, wherein the flow conditions are an enforced flow or a spontaneous flow.

### Claim 14

The method according to 1, wherein the analysis method comprising the electric analysis step is an amperometric analysis.