# US 8,784,911 B2 · Compositions for treatment and prevention of diabetic complications using Osteomeles schwerinae

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- Number: US 8,784,911 B2
- Document ID: US8784911B2
- Status: Granted
- Kind: B2
- Application number: 13/381,493
- Filed: 2009-11-05
- Published: 2012-04-26
- Granted: 2014-07-22
- Earliest claimed: 2009-11-05
- Assignees: Korea Institute of Oriental Medicine
- Applicants: Jin Sook Kim; Dae Sik Jang; Young Sook Kim; Yun Mi Lee; Joo-Hwan Kim; Eunjin Shon; Junghyun Kim; Chan-Sik Kim; Dong Ho Jung; Nan Hee Kim; Nam-Hee Yoo; Jun Lee
- Inventors: Jin Sook Kim; Dae Sik Jang; Young Sook Kim; Yun Mi Lee; Joo-Hwan Kim; Eunjin Shon; Junghyun Kim; Chan-Sik Kim; Dong Ho Jung; Nan Hee Kim; Nam-Hee Yoo; Jun Lee
- Web page: https://patentgrep.com/patent/US8784911B2

## CPC

- A61K 36/73: Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- A61P 13/12: of the kidneys
- A61P 35/00: Antineoplastic agents
- A61P 39/06: Free radical scavengers or antioxidants
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to a treatment agent for diabetic complications, and a method for the treatment of diabetic complications using the same. The present invention confirmed that Osteomeles schwerinae extract and its fractions inhibited the generation of AGEs, the index for diabetic complications, inhibited the activation of aldose reductase, had anti-cataract activity, and had anti-oxidative activity. Thus, the present invention relates to a preventive and therapeutic agent for diabetic complications containing the said Osteomeles schwerinae extract and its fraction as an active ingredient.

## Claims

### Claim 1

A method for treatment of a diabetic complication comprising:
administering a pharmaceutically effective dose of a composition comprising an extract of Osteomeles schwerinae or a fraction thereof as an active ingredient to a subject having diabetes, wherein the diabetic complication is selected from the group consisting of diabetic nephropathy, diabetic retinopathy, and diabetic cataract; wherein the extract is prepared by extracting Osteomeles schwerinae with ethanol or aqueous ethanol as an extracting solvent, and wherein the fraction is obtained by systematic sequential fractionation of the Osteomeles schwerinae extract using hexane, ethyl acetate, butanol and water in that order.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the extract is obtained from the twigs, leaves or seeds of Osteomeles schwerinae.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the fractions contains 2″-O-acetylvitexin, hyperoside or quercitrin.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the fraction is obtained by:
i) removing ethanol or aqueous ethanol solvent from the ethanol or aqueous ethanol extract of Osteomeles schwerinae to obtain a crude extract product;
ii) adding water and hexane to the extract product obtained from step (i), separating a first water layer and hexane layer and removing hexane from the hexane layer to obtain a hexane fraction;
iii) adding ethyl acetate to the first water layer obtained from step (ii), separating a second water layer and ethyl acetate layer and removing ethyl acetate from the ethyl acetate layer to obtain an ethyl acetate fraction;
iv) adding butanol to the second water layer obtained from step (iii), separating a third water layer and butanol layer and removing butanol from the butanol layer to obtain a butanol fraction; and
v) removing water from the third water layer obtained from step (iv) to obtain a water fraction.