# US 8,784,873 B2 · Use of histone deacetylase inhibitors in changing MRJP3 protein in royal jelly

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- Number: US 8,784,873 B2
- Document ID: US8784873B2
- Status: Granted
- Kind: B2
- Application number: 12/507,545
- Filed: 2009-07-22
- Published: 2011-01-27
- Granted: 2014-07-22
- Earliest claimed: 2009-07-22
- Assignees: Naturewise Biotech & Medicals Corporation
- Applicants: Chung-Yang Huang; Chia-Nan Chen; Wei-Jung Chen; Wei-Jan Huang; Li-Ling Chi
- Inventors: Chung-Yang Huang; Chia-Nan Chen; Wei-Jung Chen; Wei-Jan Huang; Li-Ling Chi
- Web page: https://patentgrep.com/patent/US8784873B2

## CPC

- A61K 31/353: 3,4-Dihydrobenzopyrans, e.g. chroman, catechin
- A01K 53/00: Feeding or drinking appliances for bees
- A61K 31/185: Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids (hydroxamic acids A61K31/16; peroxy acids A61K31/327)
- A61K 35/644: Beeswax; Propolis; Royal jelly; Honey
- Y02P 20/582: Recycling of unreacted starting or intermediate materials
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

The invention provides a method of changing a ratio of 68 to 64 kDa protein of MRJP3 in a royal jelly, a method of producing a royal jelly comprising MRJP3 having a changed ratio of 68 to 64 kDa protein relative to a control royal jelly and the royal jelly produced thereform. Also provided is a method of promoting the growth of the larva of a queen bee comprising feeding the larva of the queen bee a royal jelly of the invention. Further provided is a method of producing bee larva, pupa and queen bees with sizes larger than normal.

## Claims

### Claim 1

A method of producing bee larvae that are at least 100% greater in weight than control larvae comprising feeding young worker bees a histone deacetylase (HDAC) inhibitor or a mixture of HDAC inhibitors and feeding bee larvae royal jelly secreted by these young worker bees, while the bee larvae of the control receive the royal jelly produced by young worker bees not fed with a HDAC inhibitor or a mixture of HDAC inhibitors, wherein the HDAC inhibitor is selected from the group consisting of suberoylanilide hydroxamide acid (SAHA), Taiwanese green propolis, propolin A, propolin B, propolin C, propolin D, propolin E, propolin F, propolin G, propolin H, propolin I, propolin J, and

### Claim 2 (depends on 1)

The method of claim 1, wherein the weight of the larvae feeding on the royal jelly produced by worker bees fed with the HDAC inhibitor or the mixture of HDAC inhibitors after 72 hrs increases higher than 1.5 times.

### Claim 3 (depends on 1)

The method of claim 1, wherein the weight of the larvae feeding on the royal jelly produced by worker bees fed with the HDAC inhibitor or the mixture of HDAC inhibitors after 72 hrs increases by about 2 to 5 times.

### Claim 4 (depends on 1)

The method of claim 1, wherein the weight of the larvae feeding on the royal jelly produced by worker bees fed with the HDAC inhibitor or the mixture of HDAC inhibitors after 72 hrs increases by about 3 to 5 times.

### Claim 5 (depends on 1)

The method of claim 1, wherein the royal jelly has a changed ratio of 68 to 64 kDa protein of major royal jelly proteins 3 (MRJP3) from 4:6 to 8:2.

### Claim 6

A method of producing bee larvae that are at least 100% greater in weight than control larvae comprising feeding young worker bees a histone deacetylase (HDAC) inhibitor or a mixture of HDAC inhibitors and feeding bee larvae royal jelly secreted by these young worker bees, while the bee larvae of the control receive the royal jelly produced by young worker bees not fed with a HDAC inhibitor or a mixture of HDAC inhibitors, wherein the HDAC inhibitor is selected from the group consisting of:

### Claim 7 (depends on 1)

A method of claim 1, wherein the bee larvae can be fed by worker bees on the royal jelly secreted therefrom or by human with the royal jelly collected from worker bees.