# US 8,784,848 B2 · Method for preparing a stable oil-in-water emulsion

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- Number: US 8,784,848 B2
- Document ID: US8784848B2
- Status: Granted
- Kind: B2
- Application number: 13/379,652
- Filed: 2010-06-22
- Published: 2012-07-19
- Granted: 2014-07-22
- Earliest claimed: 2009-06-24
- Assignees: Genialis
- Applicants: Isabelle Desjardins-Lavisse; Stéphane Desobry
- Inventors: Isabelle Desjardins-Lavisse; Stéphane Desobry
- Web page: https://patentgrep.com/patent/US8784848B2

## CPC

- A61P 17/00: Drugs for dermatological disorders
- B01F 23/4111: {using vibrations}
- B01F 23/4105: {Methods of emulsifying}
- B01F 31/85: with a vibrating element inside the receptacle
- B01F 23/4143: {Microemulsions}
- B01F 23/4145: {Emulsions of oils, e.g. fuel, and water}
- B01F 2215/0454: Numerical frequency values
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- B01F: MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING (mixing paints B44D3/06)

## Abstract

The invention relates to a method for preparing a stable oil-in-water emulsion without adding an emulsifier. According to the method, a mixture of a lipid phase and an aqueous phase is subjected to vibrating energy, in a sealed container, by applying a transducer operating at a frequency of more than 900 kHz.

## Claims

### Claim 1

A method of preparing a stable oil-in-water emulsion comprising combining a lipid or lipophilic phase with an aqueous phase, and exposing the mixture to vibratory energy in a closed receptacle by application of one or more transducers, wherein said one or more transducers operate only in a frequency range of between 900 kHz and 3 MHz.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the vibratory treatment is applied under substantially cavitation-free conditions.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the transducer operates at a frequency of less than 1,500 kHz.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the transducer operates at a frequency greater than 1 MHz.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the mixture, or emulsion, contains no added emulsifier.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the transducer is of the piezoelectric type.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the vibratory energy is applied to the mixture in the lower part of the closed receptacle containing the mixture.

### Claim 8 (depends on 1)

The method according to claim 1, wherein the lipid phase constitutes no more than 40% of the total weight.

### Claim 9 (depends on 1)

The method according to claim 1, wherein the mixture is kept within a predetermined temperature range.

### Claim 10 (depends on 1)

The method according to claim 1, wherein the vibratory treatment is continued until the lipid phase is completely dispersed, the final desired lipid phase concentration is obtained or the final desired particle size is obtained.

### Claim 11 (depends on 1)

The method according to claim 1, wherein the vibratory treatment is continued until a micrometric particle size is obtained.

### Claim 12 (depends on 1)

The method according to claim 1, wherein the emulsion obtained is stable for several weeks to several months at ambient temperature.

### Claim 13 (depends on 1)

The method according to claim 1 wherein the lipid phase constitutes no more than 30% of the total weight.

### Claim 14 (depends on 1)

The method according to claim 1 wherein the lipid phase constitutes no more than 20% of the total weight.

### Claim 15 (depends on 9)

The method according to claim 9 wherein the predetermined temperature range is between 20 and 30° C.

### Claim 16 (depends on 10)

The method according to claim 10 wherein the vibratory treatment is continued until a discontinuous phase is obtained which has particles of an average diameter of less than 50 μm.

### Claim 17 (depends on 10)

The method according to claim 10 wherein the vibratory treatment is continued until a discontinuous phase is obtained which has particles of an average diameter of less than 20 μm.

### Claim 18 (depends on 1)

The method according to claim 1, wherein the vibratory treatment is continued until a nanometric particle size is obtained.