# US 8,784,835 B2 · Method for producing muscimol and/or reducing ibotenic acid from amanita tissue

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- Number: US 8,784,835 B2
- Document ID: US8784835B2
- Status: Granted
- Kind: B2
- Application number: 13/933,861
- Filed: 2013-07-02
- Published: 2014-01-02
- Granted: 2014-07-22
- Earliest claimed: 2012-07-02
- Applicants: Trent Austin
- Inventors: Trent Austin
- Web page: https://patentgrep.com/patent/US8784835B2

## CPC

- A61K 36/07: Basidiomycota, e.g. Cryptococcus
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A method for producing muscimol and or/reducing ibotenic acid from Amanita tissue, and or producing a nutritional supplement therefrom.

## Claims

### Claim 1

A method for producing a liquid dietary supplement from Amanita tissue, the method comprising:
a. providing tissue comprising an Amanita fungus comprising ibotenic acid within the tissue;
b. providing a reactant comprising an enzymatically effective amount of glutamate decarboxylase;
c. combining the tissue and reactant such that the ratio of muscimol to ibotenic acid increases; and
d. adding the reaction product of step (c) to a beverage to make the liquid dietary supplement.

### Claim 2 (depends on 1)

The method of claim 1, further comprising comminuting and drying the Amanita tissue prior to combining the tissue and reactant.

### Claim 3 (depends on 2)

The method of claim 2, wherein the method further comprises rehydrating the tissue prior to combining the tissue and reactant.

### Claim 4 (depends on 3)

The method of claim 3, further comprising heating the tissue and reactant to a temperature of at least 175° F.

### Claim 5 (depends on 4)

The method of claim 4, further comprising heating the tissue and reactant to a temperature of at least 175° F. for at least about one hour.

### Claim 6 (depends on 4)

The method of claim 4, wherein the tissue and reactant are reacted at a pH below 7.0.

### Claim 7 (depends on 6)

The method of claim 6, further comprising filtering the resultant product.

### Claim 8 (depends on 7)

The method of claim 7, wherein the filtering occurs through activated carbon.

### Claim 9 (depends on 1, 4)

The method of claim 4, wherein the resultant product has a ratio of muscimol to ibotenic acid that is at least 300 times greater than this ratio in the tissue of claim 1, step (a).

### Claim 10 (depends on 4)

The method of claim 4, wherein the resultant product has a muscimol to ibotenic acid ratio of at least 75 to 1.

### Claim 11 (depends on 1)

The method of claim 1, wherein the tissue comprises Amanita muscaria.

### Claim 12

A method for producing a liquid dietary supplement from Amanita tissue, the method comprising:
a. providing tissue comprising an Amanita fungus comprising ibotenic acid within the tissue;
b. comminuting and drying the tissue;
c. reconstituting the tissue of step (b);
d. subjecting the reconstituted tissue of step (c) to a pH below 4.5;
e. subjecting the reconstituted tissue of step (d) to heat above 175° F.; and
f. adding the reconstituted tissue of step (e) to a beverage to make the liquid dietary supplement.