# US 8,784,831 B2 · P. gingivalis antigenic composition

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- Number: US 8,784,831 B2
- Document ID: US8784831B2
- Status: Granted
- Kind: B2
- Application number: 13/566,264
- Filed: 2012-08-03
- Published: 2013-06-27
- Granted: 2014-07-22
- Earliest claimed: 1999-12-24
- Assignees: CSL Limited; The University of Melbourne
- Applicants: Eric Charles Reynolds; Nada Slakeski; Chao Guang Chen; Ian George Barr
- Inventors: Eric Charles Reynolds; Nada Slakeski; Chao Guang Chen; Ian George Barr
- Web page: https://patentgrep.com/patent/US8784831B2

## CPC

- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61P 1/02: Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
- A61P 31/04: Antibacterial agents
- A61P 37/04: Immunostimulants
- A61Q 11/00: Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
- C07K 14/195: from bacteria
- C07K 16/12: against material from bacteria
- C07K 16/1203: Gram-negative bacteria
- C07K 16/1257: {from Bacteridaceae (F)}
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- A61K 2039/505: {comprising antibodies}
- A61K 2800/86: Products or compounds obtained by genetic engineering
- C07K 2319/00: Fusion polypeptide
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention provides an antigenic composition, the composition comprising at least one recombinant protein. The recombinant protein comprises at least one epitope. The epitope is reactive with an antibody which is reactive with a polypeptide having the sequence set out in SEQ. ID. NO. 3 or SEQ. ID. NO. 5. The invention also provides methods and compositions for the production of the recombinant protein. Also provided are methods for the diagnosis, treatment and prevention of P. gingivalis infection.

## Claims

### Claim 1

An antigenic composition comprising at least one isolated and purified protein, wherein the isolated and purified protein consists of SEQ ID NO: 5 or a fragment thereof capable of raising an immune response against P. gingivalis, and wherein said protein is not complexed with other Arg-specific and Lys-specific P. gingivalis proteinase and adhesin proteins.

### Claim 2 (depends on 1)

The antigenic composition as claimed in claim 1 wherein the antigenic composition further comprises an adjuvant.

### Claim 3

An antigenic composition comprising a chimeric protein, wherein the chimeric protein comprises a first and a second polypeptide, wherein the first polypeptide consists of SEQ ID NO: 5 or a fragment thereof capable of raising an immune response against P. gingivalis, and the second polypeptide comprises a P. gingivalis sequence.

### Claim 4 (depends on 3)

The antigenic composition as claimed in claim 3, wherein the antigenic composition further comprises an adjuvant.

### Claim 5 (depends on 1)

A method of reducing the severity of P. gingivalis infection in a subject, the method comprising administering to the subject an antigenic composition as claimed in claim 1.

### Claim 6 (depends on 3)

A method of reducing the severity of P. gingivalis infection in a subject, the method comprising administering to the subject the antigenic composition as claimed in claim 3.

### Claim 7 (depends on 1)

The antigenic composition as claimed in claim 1 wherein the isolated and purified protein consists of SEQ ID NO: 5.