# US 8,784,813 B2 · L-amino acid oxidase with cytotoxic activity from Aplysia punctata

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- Number: US 8,784,813 B2
- Document ID: US8784813B2
- Status: Granted
- Kind: B2
- Application number: 12/163,261
- Filed: 2008-06-27
- Published: 2009-01-29
- Granted: 2014-07-22
- Earliest claimed: 2003-01-20
- Assignees: Thomas Rudel
- Applicants: Daniel Butzke; Sigrid Goedert; Michael Dittrich; Thomas Rudel; Thomas Meyer
- Inventors: Daniel Butzke; Sigrid Goedert; Michael Dittrich; Thomas Rudel; Thomas Meyer
- Web page: https://patentgrep.com/patent/US8784813B2

## CPC

- C07K 14/43504: {from invertebrates}
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- C12N 9/0022: {with oxygen as acceptor (1.4.3)}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a cytotoxic polypeptide which is an L-amino acid oxidase isolated from the ink of the sea hare Aplysia punctata.

## Claims

### Claim 1

A method of treating cancer comprising the step of administering to a subject in need thereof a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2, 4, or 6.

### Claim 2 (depends on 1)

The method according to claim 1 wherein said cancer is selected from the group consisting of lung cancer, breast cancer, prostate cancer, colon cancer, cervix cancer, uterus cancer, larynx cancer, stomach cancer, liver cancer, Ewings sarcoma, acute lymphoid leukemia, chronic myeloid leukemia, apoptosis resistant leukemia, MDR lung cancer, pancreas cancer, gastric cancer, kidney cancer, gliomas, melanomas, chronic lymphoid leukemia, and lymphoma.

### Claim 3

A method of treating cancer comprising the step of administering to a subject in need thereof a composition comprising an effective amount of a polypeptide that comprises the amino acid sequence shown in SEQ ID NO: 2, 4, or 6 and a diluent, a carrier and/or an adjuvant.

### Claim 4 (depends on 1)

The method of claim 1, wherein the polypeptide is produced recombinantly.

### Claim 5 (depends on 4)

The method of claim 4, wherein the polypeptide is produced by a host cell selected from the group consisting of a bacterial cell, a yeast cell, an insect cell, and a mammalian cell.

### Claim 6 (depends on 1)

The method of claim 1, wherein the subject is a human subject.

### Claim 7 (depends on 3)

The method of claim 3, wherein the composition is administered by a route of administration selected from the group consisting of oral, nasal, intravenous, intraperitoneal, intramuscular, and subcutaneous.

### Claim 8 (depends on 3)

The method of claim 3, further comprising the step of administering a compound selected from the group consisting of L-lysine, L-arginine, a flavine nucleoside, and a mixture thereof.

### Claim 9 (depends on 3)

The method according to claim 3, wherein said cancer is selected from the group consisting of lung cancer, breast cancer, prostate cancer, colon cancer, cervix cancer, uterus cancer, larynx cancer, stomach cancer, liver cancer, Ewings sarcoma, acute lymphoid leukemia, chronic myeloid leukemia, apoptosis resistant leukemia, MDR lung cancer, pancreas cancer, gastric cancer, kidney cancer, gliomas, melanomas, chronic lymphoid leukemia, and lymphoma.

### Claim 10 (depends on 3)

The method of claim 3, wherein the subject is a human subject.

### Claim 11 (depends on 1)

The method of claim 1, wherein the cancer is selected from the group consisting of lung cancer and melanoma.

### Claim 12 (depends on 1)

The method of claim 1, wherein the cancer is selected from the group consisting of leukemia and liver cancer.