# US 8,784,734 B2 · Reusable sheaths for separating magnetic particles > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,784,734 B2 - Document ID: US8784734B2 - Status: Granted - Kind: B2 - Application number: 12/783,619 - Filed: 2010-05-20 - Published: 2011-11-24 - Granted: 2014-07-22 - Earliest claimed: 2010-05-20 - Assignees: Abbott Laboratories - Applicants: Douglas M. Campbell; Gregory E. Gardner - Inventors: Douglas M. Campbell; Gregory E. Gardner - Web page: https://patentgrep.com/patent/US8784734B2 ## CPC - G01N 35/0098: {involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C)} - B03C 1/01: by addition of magnetic adjuvants - B03C 1/03: rotating, e.g. of the carousel type - B01J 2219/00452: Means for the recovery of reactants or products - B01J 2219/005: Beads - B01J 2219/00648: by the use of solid beads - B01J 2219/00655: the compounds being bound to magnets embedded in or on the solid supports - B03C 2201/18: Magnetic separation whereby the particles are suspended in a liquid - G01N 2035/00574: {Means for distributing beads} - G01N 2035/1088: {Coaxial with a carousel} - Y10T 436/25: including sample preparation - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B) - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract An assembly that utilizes reusable sheaths for covering magnetic rods for collecting particles in an inverse magnetic particle process. The magnetic rod is removed from the reusable sheath to release particles at the same or a different location. The reusable sheaths can be assembled in a cylindrical plate, which can be rotated to position a clean sheath for each step of the inverse magnetic particle process. When not being used for particle separation, the sheaths can be washed of potentially contaminating solution in wash receptacles. ## Claims ### Claim 1 An apparatus comprising: a post having a longitudinal axis; a retainer comprising a plate having a plurality of sheath apertures radially spaced from a center of the retainer and a post aperture to receive the post, wherein the retainer is rotatably coupled to the post and movable along the longitudinal axis of the post; and a plurality of sheaths removably disposed within the sheath apertures to depend from the retainer. ### Claim 2 (depends on 1) The apparatus of claim 1, wherein the plate comprises teeth on a peripheral edge of the plate. ### Claim 3 (depends on 2) The apparatus of claim 2 further comprising a drive to engage the teeth to rotate the retainer relative to the post. ### Claim 4 (depends on 3) The apparatus of claim 3, wherein the drive comprises one of a Geneva mechanism, a belt drive or a sprocket drive. ### Claim 5 (depends on 1) The apparatus of claim 1 further comprising a processing head to move the retainer in a substantially vertical direction along the post. ### Claim 6 (depends on 5) The apparatus of claim 5, wherein the processing head includes a first lifter coupled to a major surface of the plate. ### Claim 7 (depends on 5) The apparatus of claim 5 further comprising a magnetic rod, wherein the processing head is to move the magnetic rod into any one of the sheaths. ### Claim 8 An apparatus comprising: a post having a longitudinal axis; a plate having a first major surface, a second major surface opposite the first major surface, and a third surface between the first major surface and the second major surface, the third surface having a plurality of teeth, the plate having a plurality of sheath apertures and a post aperture extending through the first and second major surfaces thereof, the post aperture to receive the post, wherein the plate is rotatable about the longitudinal axis of the post and movable along the longitudinal axis of the post; and a plurality of sheaths having an open top end and a closed bottom end, the sheaths to be removably disposed within the sheath apertures to depend downwardly from the plate. ### Claim 9 (depends on 8) The apparatus of claim 8, wherein the plate is substantially cylindrical shaped. ### Claim 10 (depends on 8) The apparatus of claim 8, wherein the sheath apertures are spaced radially on the plate. ### Claim 11 (depends on 8) The apparatus of claim 8 further comprising a gear drive to engage the teeth to rotate the plate. ### Claim 12 (depends on 11) The apparatus of claim 11, wherein the gear drive comprises a stepper motor. ### Claim 13 (depends on 8) The apparatus of claim 8, wherein the sheaths are retained within the sheath apertures via a friction fit. ### Claim 14 (depends on 8) The apparatus of claim 8, wherein the sheaths comprise collars formed at the open top ends of the sheaths, the collars to retain the sheaths within the sheath apertures. ### Claim 15 (depends on 8) The apparatus of claim 8 further comprising: rings formed near the open top ends of the sheaths that fit within slots in the sheath apertures, the rings to retain the sheaths within the sheath apertures; or rings formed within walls of the sheath apertures that fit into slots near the open top ends of the sheaths, the rings to retain the sheaths within the sheath apertures. ### Claim 16 An apparatus comprising: a post having a longitudinal axis; a sheath retainer to hold a plurality of sheaths, the sheath retainer comprising a plate having a plurality of sheath apertures spaced radially on the plate and an aperture to receive the post; a plurality of sheaths to receive a magnetic rod, each of the sheaths having an open end and closed end, the sheaths removably disposed within the sheath apertures; a processing head to move the sheath retainer along the longitudinal axis of the post; and a drive to rotate the sheath retainer relative to the post, wherein the processing head and the drive are to move the sheath retainer between a first position where the closed end of a first sheath is disposed within a first container and a second position where the closed end of the first sheath is disposed within a second container. ### Claim 17 (depends on 16) The apparatus of claim 16, wherein the first container is a reaction vessel. ### Claim 18 (depends on 17) The apparatus of claim 17, wherein the second container is a wash receptacle. ### Claim 19 (depends on 16) The apparatus of claim 16 further comprising a second sheath, wherein the second sheath is disposed within the second container when the sheath retainer is in the first position. ### Claim 20 (depends on 19) The apparatus of claim 19, wherein the second sheath is disposed within the first container when the sheath retainer is in the second position. ### Claim 21 (depends on 6) The apparatus of claim 6 further comprising a guide plate having a second post aperture to receive the post and a magnetic rod depending from the guide plate. ### Claim 22 (depends on 21) The apparatus of claim 21 further comprising a second lifter coupled to the guide plate to move the guide plate along the longitudinal axis of the post. ### Claim 23 (depends on 22) The apparatus of claim 22, wherein the processing head is to control the first lifter and the second lifter.