# US 8,784,659 B2 · Method for controlling microbial biofilm in aqueous systems

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- Number: US 8,784,659 B2
- Document ID: US8784659B2
- Status: Granted
- Kind: B2
- Application number: 12/892,490
- Filed: 2010-09-28
- Published: 2011-03-03
- Granted: 2014-07-22
- Earliest claimed: 2007-08-08
- Assignees: General Electric Company
- Applicants: Wilson Kurt Whitekettle; Gloria Jean Tafel; Qing Zhao; Linna Wang; Dorothy Reynolds; Paul Frail; Juan Jiang; David M. Polizzotti
- Inventors: Wilson Kurt Whitekettle; Gloria Jean Tafel; Qing Zhao; Linna Wang; Dorothy Reynolds; Paul Frail; Juan Jiang; David M. Polizzotti
- Web page: https://patentgrep.com/patent/US8784659B2

## CPC

- C02F 1/50: by addition or application of a germicide or by oligodynamic treatment {(C02F1/4606, C02F1/467, C02F1/76 take precedence)}
- A01N 47/44: Guanidine; Derivatives thereof
- A01N 47/48: containing —S—C≡N groups (A01N43/00 - A01N47/38 take precedence)
- C02F 2303/04: Disinfection
- C02F 2303/20: Prevention of biofouling
- C02F 2305/04: Surfactants, used as part of a formulation or alone
- C02F 2305/14: Additives which dissolves or releases substances when predefined environmental conditions are reached, e.g. pH or temperature
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

A process has been found which increases the efficiency and effectiveness of introducing antimicrobial compounds into complex biofilm matrices through the use of liposome carriers, thereby removing the biofouling in industrial water bearing systems, including piping, heat exchanges, condensers, filtration systems and fluid storage tanks. According to one embodiment of the invention, antimicrobial compound containing liposomes are added to water systems prone to biofouling and biofilm formation. The liposomes, being similar in composition to microbial membranes or cells, are readily incorporated into the existing biofilm. Once the antimicrobial compound containing liposomes become entrained with the biofilm matrix, the decomposition or programmed disintegration of the liposome proceeds. Thereafter, the biocidal aqueous core is released to react directly with the biofilm encased microorganisms. Upon the death of the organisms, the polysaccharide/protein matrix decomposes and thereby results in reduced fouling of the water bearing system, resulting in increased heat transfer, increased flux, less deposit of colloidal and particulate solids and dissolved organics on the surface of the microfiltration membrane, thereby reducing the frequency and duration of the membrane cleaning and ultimate replacement.

## Claims

### Claim 1

A process for controlling fouling of an aqueous system including a biofilm matrix and microorganisms in contact with said matrix, said process comprising:
providing a biofilm control agent in a vesicle, wherein said vesicle is prepared from lecithin and/or multiblock polymers and wherein said vesicle contains a stabilizing agent wherein said stabilizing agent is a buffer comprising a mixture of two or more compounds selected from the group consisting of a citrate salt, a chlorate salt and an acetate salt; and
adding said vesicle to said aqueous system whereby said vesicle is incorporated into said biofilm and releases said biofilm control agent to contact said microorganisms.

### Claim 2 (depends on 1)

Process as recited in claim 1 wherein said stabilizing agent is a buffer comprising a mixture of two or more compounds selected from the group consisting of the metal salt of a citrate/chlorate buffer, a metal salt of an acetate/chlorate buffer, and a citrate/acetate buffer.

### Claim 3 (depends on 1)

Process as recited in claim 1 wherein said buffer is selected from the group consisting of a sodium citrate buffer, a sodium acetate buffer, a sodium citrate/sodium chlorate buffer mixture, and a sodium acetate/sodium chlorate buffer mixture.

### Claim 4 (depends on 1)

The process of claim 1, wherein said multiblock polymers contain hydrophobic and hydrophilic blocks.

### Claim 5

A process for controlling fouling of an aqueous system including a biofilm matrix and microorganisms in contact with said matrix, said process comprising:
providing a biofilm control agent in a vesicle, said vesicle containing a stabilizing agent wherein said stabilizing agent is a buffer comprising a mixture of two or more compounds selected from the group consisting of a citrate salt, a chlorate salt and an acetate salt; and
adding said vesicle to said aqueous system whereby said vesicle is incorporated into said biofilm and releases said biofilm control agent to contact said microorganisms.

### Claim 6 (depends on 5)

Process as recited in claim 5 wherein said stabilizing agent is a buffer comprising a mixture of two or more compounds selected from the group consisting of the metal salt of a citrate/chlorate buffer, a metal salt of an acetate/chlorate buffer, and a citrate/acetate buffer.

### Claim 7 (depends on 5)

Process as recited in claim 5 wherein said buffer is selected from the group consisting of a sodium citrate buffer, a sodium acetate buffer, a sodium citrate/sodium chlorate buffer mixture, and a sodium acetate/sodium chlorate buffer mixture.

### Claim 8 (depends on 5)

Process as recited in claim 5 wherein said biofilm control agent comprises a biocide or non-biocidal agent.

### Claim 9 (depends on 8)

Process as recited in claim 8 wherein said biofilm control agent is a biocide and said biocide is a non-oxidizing, oxidizing, or molluscicide antimicrobial compound.

### Claim 10 (depends on 8)

Process as recited in claim 8 wherein said biofilm control agent is a biocide and said biocide is chosen from the group consisting of 2-bromo-2-nitropropane-1,3-diol, 5-chloro-2-methyl-4-isothiazolin-3-one/2-methyl-4-isothiazolin-3-one, n-alkyl-dimethylbenzylammonium chloride, 2,2-dibromo-3-nitrilopropionamidemethylene-bis(thiocyanate), dodecylguanidine hydrochloride, glutaraldehyde, 2-(tert-butylamino)-4-chloro-6-(ethylamino)-s-triazine, beta-bromonitrostyrene, tributyltinoxide, n-tributyltetradecyl phosphonium chloride, tetrahydroxymethyl phosphonium salt, 4,5-dichloro-1,2-dithio-3-one, sodium dimethyldithiocarbamate, disodium ethylenebisdithiocarbamate, Bis(trichloromethyl) sulfone, 3,5-dimethyl-tetrahydro-2H-1,3,5-thiadiazine-2-thione, 1,2-benzisothiazolin-3-one, decylthioethylamine hydrochloride, copper sulfate, silver nitrate, colloidal silver, bromochlorodimethylhydantoin, sodium bromide, dichlorodimethylhydantoin, sodium hypochlorite, hydrogen peroxide, chlorine dioxide, sodium chlorite, bromine chloride, peracetic acid and precursors, sodium trichloroisocyanurate, sodium trichloroisocyanurate, dibromo, dicyano butane and combinations thereof.

### Claim 11 (depends on 8)

Process as recited in claim 8 wherein said biofilm control agent is a non-biocidal agent, said non-biocidal agent being chosen from the group consisting of biodispersants, ethylene oxide / propylene oxide copolymers, trichlorohexanoic acid, polysiloxanes, carbosilanes, polyethyleneimine, bacteria, microorganisms, plasmids, phagocytes, macrophages, toxin-producing microorganisms, amino acids, proteins, peptides, DNA, RNA, base pairs, antisense RNA, pharmaceuticals, antibiotics, chelators, natural extracts, organic/inorganic redox agents, organic and inorganic dye sensitizers, apoptosis signaling reagent, microorganism- and plant-derived extracts and by-products, metabolic components, preservatives, toxic phytochemicals, microbial toxins, catalysts that generate free radicals or active oxygen species, L-cystin and enzymes or combinations thereof.

### Claim 12 (depends on 5)

Process as recited in claim 5 wherein said vesicle is composed of a liposome.

### Claim 13 (depends on 5)

The process of claim 5 wherein said vesicle is produced in sizes from about 0.05 to about 15μ.

### Claim 14 (depends on 13)

The process of claim 13 wherein said vesicle is produced in sizes from about 0.1 to about 10μ.

### Claim 15 (depends on 5)

The process of claim 5 wherein said vesicle is a liposome vesicle and said biofilm control agent is incorporated into said liposome vesicle in an amount of about 0.05 to about 5.0 grams control agent active per gram lipid.

### Claim 16 (depends on 15)

The process of claim 15 wherein said biofilm control agent is incorporated into said liposome in an amount of from 0.1 to about 2.0 grams biofilm control agent active per gram lipid.

### Claim 17 (depends on 5)

The process of claim 5 wherein said vesicle is a liposome vesicle and wherein said biofilm control agent is added to said aqueous system in an amount of from about 0.01 ppm to about 100 ppm.

### Claim 18 (depends on 5)

The process of claim 5 wherein said aqueous system is chosen from the group consisting of water distribution systems, cooling towers, boiler systems, showers, aquaria, sprinklers, spas, cleaning baths systems, air washers, pasteurizers, air conditioners, fluid transporting pipelines, storage tanks, ion exchange resins, food and beverage processing lines, metalworking fluid baths, coal and mineral slurries, metal leaching fluids, wastewater treatment facilities, mollusk control, acid mine drainage, or any application prone to biofouling by microbial species.

### Claim 19 (depends on 5)

The process of claim 5 wherein said aqueous system is chosen from the group consisting of anti-corrosion treatments, hormone, vitamin or anti-oxidant treatments, antibiotic and gene therapies, pesticides for agriculture and commercial home uses, food additives and preservatives, chemical and biological detection, color and flavor enhancement, odor control and aquatic pest management.