# US 8,784,623 B2 · Nanopore device and a method for nucleic acid analysis

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- Number: US 8,784,623 B2
- Document ID: US8784623B2
- Status: Granted
- Kind: B2
- Application number: 13/003,308
- Filed: 2009-02-17
- Published: 2011-05-12
- Granted: 2014-07-22
- Earliest claimed: 2008-07-17
- Assignees: Koninklijke Philips N.V.
- Applicants: Pieter Jan Van Der Zaag; Anja Van De Stolpe; Elaine McCoo; Eva Van Van Wanrooij
- Inventors: Pieter Jan Van Der Zaag; Anja Van De Stolpe; Elaine McCoo; Eva Van Van Wanrooij
- Web page: https://patentgrep.com/patent/US8784623B2

## CPC

- G01N 33/48721: {Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68)}
- Y10T 29/49002: Electrical device making
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A nanopore device is described wherein is provided a sample input (110), an input chamber (120), and first and second sample chambers (130, 140) connected to the input chambers (120) via first and second nanopores (135, 145).

## Claims

### Claim 1

A nanopore device, arranged to cooperate with a sample of nucleic acid, the nanopore device comprising:
an input chamber, for containment of the sample of nucleic acid;
a first chamber connected to the input chamber by a first nanopore for detecting a first target molecule;
means to apply an electrical potential difference across the first nanopore, the electrical potential being arranged to draw a first fragment of the sample of nucleic acid through the first nanopore; and
an electrolytic solution which is capable of flow through the first nanopore under the influence of the electrical potential difference, the flow detectable as a first ionic current, changes in the first ionic current being indicative of detection of the first target molecule wherein, the nanopore device further comprises:
a second chamber connected to the input chamber by a second nanopore for detecting a second target molecule, the second target molecule being different from the first target molecule;
the means to apply an electrical potential difference being arranged to apply an electrical potential difference across the second nanopore which is equal to, and common with, the electrical potential difference across the first nanopore;
the electrical potential across the second nanopore being arranged to draw a second fragment of the sample of nucleic acid through the second nanopore and to influence the electrolytic solution to flow through the second nanopore; and
the flow being detectable as a second ionic current, changes in the second ionic current being indicative of detection of a second target molecule by the second nanopore.

### Claim 2 (depends on 1)

A nanopore device as claimed in claim 1 further comprising a third chamber connected to the input chamber by a third nanopore, an electrical potential difference being applied across the third nanopore equal to, and common with, the electrical potential difference across the first nanopore, the electrical potential across the third nanopore being arranged to draw a third fragment of the sample of nucleic acid through the third nanopore and to influence the electrolytic solution to flow through the third nanopore, the flow detectable as a third ionic current by the current detector, changes in the third ionic current being indicative of detection of a control target molecule for validating device measurement.

### Claim 3 (depends on 1)

A nanopore device as claimed in claim 1 further comprising a correlating means arranged to correlate detections indicated by changes in at least two of the ionic currents.

### Claim 4 (depends on 1)

A nanopore device as claimed in claim 1 wherein the first target molecule is a selected wild gene sequence and the second target molecule is a specific mutation in the same gene sequence, for relative detection of the statistical occurrence of the mutation.

### Claim 5 (depends on 1)

A nanopore device as claimed in claim 1 wherein at least one of the nanopores is a biological nanopore.

### Claim 6 (depends on 5)

A nanopore device as claimed in claim 5 wherein the biological nanopore is arranged in cooperation with a lipid bilayer.