# US 8,784,499 B2 · Preparation of regenerative tissue scaffolds > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,784,499 B2 - Document ID: US8784499B2 - Status: Granted - Kind: B2 - Application number: 13/070,891 - Filed: 2011-03-24 - Published: 2011-09-29 - Granted: 2014-07-22 - Earliest claimed: 2010-03-25 - Assignees: LifeCell Corporation - Applicants: Rick Owens; Wendell Sun; Mike Liu; Yong Mao - Inventors: Rick Owens; Wendell Sun; Mike Liu; Yong Mao - Web page: https://patentgrep.com/patent/US8784499B2 ## CPC - A61F 2/0063: {Implantable repair or support meshes, e.g. hernia meshes} - A61L 27/3633: {Extracellular matrix [ECM]} - A61L 27/48: with macromolecular fillers - A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like - A61P 19/00: Drugs for skeletal disorders - A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C) - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract Devices and methods for treating or repairing a tissue or organ defect or injury are provided. The devices can include tissue scaffolds produced from acellular tissue matrices and polymers, which have a stable three-dimensional shape and elicit a limited immunologic or inflammatory response. ## Claims ### Claim 1 A tissue scaffold, comprising: an acellular tissue matrix (ATM) comprising a group of ATM particulates; and a polymer; wherein the ATM particulates are encased in the polymer to form a stable three-dimensional tissue scaffold for tissue regeneration, and the tissue scaffold has a reduced immunological or inflammatory response when implanted in a human than the polymer alone; and the polymer has been dissolved in a solvent to form a polymer solution, the ATM particulates have been submerged in and mixed with the polymer solution to form a moldable mixture, and the moldable mixture has been dried to produce a polymer scaffold completely surrounding the ATM particulates. ### Claim 2 (depends on 1) The tissue scaffold of claim 1, wherein the polymer comprises a synthetic polymer. ### Claim 3 (depends on 1) The tissue scaffold of claim 1, wherein the polymer comprises a polycaprolactone. ### Claim 4 (depends on 1) The tissue scaffold of claim 1, wherein the polymer is a poly4-hydroxybutyrate. ### Claim 5 (depends on 1) The tissue scaffold of claim 1, wherein the polymer comprises a benzyl ester derivative of hyaluronic acid. ### Claim 6 (depends on 1) The tissue scaffold of claim 1, wherein the polymer comprises chitosan. ### Claim 7 (depends on 1) The tissue scaffold of claim 1, wherein the ATM comprises a dermal ATM. ### Claim 8 (depends on 7) The tissue scaffold of claim 7, wherein the dermal ATM is a human tissue matrix. ### Claim 9 (depends on 7) The tissue scaffold of claim 7, wherein the dermal ATM is a porcine tissue matrix. ### Claim 10 (depends on 1) The tissue scaffold of claim 1, wherein the ATM is a cartilage tissue matrix. ### Claim 11 (depends on 10) The tissue scaffold of claim 10, wherein the cartilage tissue matrix comprises a human cartilage. ### Claim 12 (depends on 10) The tissue scaffold of claim 10, wherein the cartilage tissue matrix comprises a porcine cartilage. ### Claim 13 (depends on 1) The tissue scaffold of claim 1, wherein the ATM is a bone tissue matrix. ### Claim 14 (depends on 13) The tissue scaffold of claim 13, wherein the bone tissue matrix is derived from human bone. ### Claim 15 (depends on 13) The tissue scaffold of claim 13, wherein the bone tissue matrix is derived from porcine bone. ### Claim 16 (depends on 1) The tissue scaffold of claim 1, wherein the ATM includes tissue matrices derived from two or more different types of tissues. ### Claim 17 (depends on 16) The tissue scaffold of claim 16, wherein the two or more different types of tissues include cartilage and demineralized bone. ### Claim 18 (depends on 1) The tissue scaffold of claim 1, wherein the solvent includes at least one of dioxane, N-methyl-2-pyrrolidone, DMSO, and acetic acid. ### Claim 19 (depends on 1) The tissue scaffold of claim 1, wherein drying includes a drying process selected from at least one of freeze drying, air drying, heated drying, drying in an argon or nitrogen atmosphere, and vacuum assisted drying. ### Claim 20 A tissue scaffold, comprising: an acellular tissue matrix (ATM) comprising a group of ATM particulates; and a polymer; wherein the ATM particulates are encased in the polymer to form a stable three-dimensional tissue scaffold for tissue regeneration, and the tissue scaffold has a reduced immunological or inflammatory response when implanted in a human than the polymer alone; and wherein the ATM particulates have been mixed with a polymer solution to form a moldable mixture, and the moldable mixture has been dried to produce the stable three-dimensional tissue scaffold. ### Claim 21 (depends on 20) The tissue scaffold of claim 20, wherein the ATM comprises a dermal ATM. ### Claim 22 (depends on 21) The tissue scaffold of claim 21, wherein the dermal ATM is a human tissue matrix. ### Claim 23 (depends on 21) The tissue scaffold of claim 21, wherein the dermal ATM is a porcine tissue matrix.