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US 8,779,312 B2Granted
Method and device for analyzing biomolecules with track-etched polymeric layers
AssigneesThe United States of America/NIH, 20/20 GeneSystems, Inc.
InventorsVladimir Knezevic, Dan-Paul Hartmann, Michael R. Emmert-Buck
Life of this patent
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Abstract
The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
Claims6
Apparatus for detecting biomolecules from a biological sample, comprising:
- at least one porous polycarbonate membrane coated with nitrocellulose on one or both sides, said nitrocellulose having an affinity to one or more biomolecules from said sample;
- wherein the nitrocellulose coated polycarbonate membrane is track-etched, less than about 30 microns thick and has a pore size ranging from between about 0.1 to 5.0 microns, and wherein said membrane is further characterized as possessing a high affinity for biomolecules, but has a low capacity for retaining such molecules.
Dependencies are inferred from the claim text.