Microarray fabrication system and method

A method for preparing a biological microarray, comprising:

• forming an array of base pads at predetermined sites on a substrate;
• disposing a molecule binding substance over each of the base pads, thereby configuring each of the base pads to capture a single nucleic acid molecule by linking the single nucleic acid molecule to the molecule binding substance;
• and
• disposing a porous attachment layer over the base pads such that the base pads are in between the porous attachment layer and the substrate, wherein the porous attachment layer is configured to attach copies of the nucleic acid molecule, and wherein the copies of the nucleic acid molecule are obtained through amplifying the single nucleic acid molecule at each base pad.

A method for preparing a biological microarray, comprising:

• forming a polymer layer on a substrate, wherein the polymer layer comprises a poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) polymer;
• disposing a photoresist layer over the polymer layer;
• forming interstitial spaces in the photoresist layer and the polymer layer;
• and
• removing the photoresist layer to expose polymer base pads, wherein the polymer base pads are coupled to a molecule binding substance.

A method for preparing a biological microarray, comprising:

• forming a polymer layer on a surface of a substrate;
• forming a plurality of spaced apart photoresist regions on the polymer;
• contacting exposed portions of the polymer layer with a plurality of primers;
• and
• removing the photoresist regions and covered portions of the polymer layer such that a plurality of spaced apart polymer pads coupled to the plurality of primers remain on the surface of the substrate.