# US 8,772,606 B2 · Non-transgenic tomato varieties having increased shelf life post-harvest

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- Number: US 8,772,606 B2
- Document ID: US8772606B2
- Status: Granted
- Kind: B2
- Application number: 13/123,391
- Filed: 2009-10-09
- Published: 2011-11-24
- Granted: 2014-07-08
- Earliest claimed: 2008-10-10
- Assignees: Arcadia Biosciences, Inc.
- Applicants: Susan R. Hurst; Dayna L. Loeffler
- Inventors: Susan R. Hurst; Dayna L. Loeffler
- Web page: https://patentgrep.com/patent/US8772606B2

## CPC

- A01H 1/06: Processes for producing mutations, e.g. treatment with chemicals or with radiation (specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 {; process for producing transgenic plants C12N15/82})
- A01H 5/08: Fruits
- A01H 6/825: {Solanum lycopersicum [tomato]}
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A series of independent human-induced, non-transgenic mutations found in at least one non-ripening (NOR) gene of tomato; tomato plants having these mutations in at least one of their NOR genes; and a method of creating and identifying similar and/or additional mutations in the NOR gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit fruit that ripen more slowly, rot more slowly, are firmer, and have a longer shelf life post-harvest as a result of non-transgenic mutations in at least one of their NOR genes.

## Claims

### Claim 1

A non-ripening (NOR) gene from tomato comprising a human-induced, non-transgenic G2434A mutation; wherein said human-induced, non-transgenic G2434A mutation comprises a nucleotide change within the NOR gene; and wherein said nucleotide change is identified according to SEQ ID NO: 1.

### Claim 2

A tomato plant containing a human-induced, non-transgenic mutation within its NOR gene; wherein said human-induced, non-transgenic mutation is G2434A; wherein said human-induced, non-transgenic mutation comprises a nucleotide change within the NOR gene; and wherein said nucleotide change is identified according to SEQ ID NO: 1.

### Claim 3 (depends on 2)

The tomato plant of claim 2, wherein expression of said mutation in the homozygous state results in fruit that are firmer than wild type fruit and turn pink when ripened at standard storage conditions.

### Claim 4 (depends on 2)

A Fruit, a seed, a pollen grain, a plant part, or the progeny of the tomato plant of claim 2; wherein the fruit, the seed, the pollen grain, the plant part, or the progeny comprises the mutation.

### Claim 5 (depends on 4)

A food or a food product comprising the fruit of claim 4.

### Claim 6

A NOR protein from tomato comprising a W258* amino acid change; wherein said amino acid change results from a human-induced, non-transgenic mutation in the NOR gene; and wherein said amino acid change is identified according to SEQ ID NO: 2.

### Claim 7

A tomato fruit comprising a W258* amino acid change in its NOR protein; wherein said amino acid change results from a human-induced, non-transgenic mutation in the NOR gene; and wherein said amino acid change is identified according to SEQ ID NO: 2.

### Claim 8 (depends on 7)

The tomato fruit of claim 7, wherein expression of said amino acid change in its NOR protein in the homozygous state results in fruit that are firmer than wild type fruit and turn pink when ripened at standard storage conditions.

### Claim 9 (depends on 7)

A food or a food product comprising the tomato fruit of claim 7.