# US 8,772,581 B2 · Genomically multiplied rapeseed plants, compositions derived therefrom and uses of same

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- Number: US 8,772,581 B2
- Document ID: US8772581B2
- Status: Granted
- Kind: B2
- Application number: 12/741,376
- Filed: 2008-11-06
- Published: 2011-04-21
- Granted: 2014-07-08
- Earliest claimed: 2007-11-06
- Assignees: Kaiima Bio Agritech Ltd.
- Applicants: Amit Avidov; Alon Lerner
- Inventors: Amit Avidov; Alon Lerner
- Web page: https://patentgrep.com/patent/US8772581B2

## CPC

- A01H 1/08: Methods for producing changes in chromosome number
- A01H 5/10: Seeds
- A01H 6/202: {Brassica napus [canola]}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A rapeseed plant having a multiplied genome being at least as fertile as a euploid rapeseed plant isogenic to the genomically multiplied plant grown under similar conditions.

## Claims

### Claim 1

A Brassica napus rapeseed plant having a doubled amphidiploid genome being at least as fertile as a euploid Brassica napus rapeseed plant isogenic to the genomically doubled plant grown under similar conditions, wherein fertility is determined by number of seeds per plant, and wherein the genomically doubled plant is grown from genomically doubled rapeseed seed, which seed have been generated by:
(a) applying a magnetic field to seeds of the euploid Brassica napus; and
(b) contacting said seeds of the euploid Brassica napus with a microtubule polymerization inhibitor under said magnetic field for targeted delivery of said microtubule polymerization inhibitor to chromatin fibers in nuclei of said seeds.

### Claim 2 (depends on 1)

The plant of claim 1, exhibiting genomic stability for at least 2 generations.

### Claim 3 (depends on 1)

The plant of claim 1, having a seed weight per 40 seeds exceeding that of said euploid rapeseed plant.

### Claim 4

A rapeseed plant as deposited under the Budapest treaty in NCIMB Ltd. and having Accession No. NCIMB 41592 Brassica napus 187-24N.

### Claim 5 (depends on 1)

A plant part of the rapeseed plant of claim 1.

### Claim 6 (depends on 1)

Rapeseed meal produced from the plant of claim 1.

### Claim 7 (depends on 5)

THE plant part of claim 5 being a seed.

### Claim 8 (depends on 1)

An isolated regenerable cell of the rapeseed plant of claim 1.

### Claim 9 (depends on 8)

The cell of claim 8, exhibiting genomic stability for at least 2 generations in culture.

### Claim 10 (depends on 8)

The cell of claim 8 being from a meristem, pollen, a leaf, a root, a root tip, an anther, a pistil, a flower, a seed or a stem.

### Claim 11 (depends on 8)

A tissue culture comprising the regenerable cell of claim 8.

### Claim 12 (depends on 1)

A method of producing seeds of rapeseed, comprising self-breeding or cross-breeding the plant of claim 1.

### Claim 13 (depends on 1)

A method of producing rapeseed oil, the method comprising:
(a) harvesting seeds of the rapeseed plant of claim 1; and
(b) processing said seeds so as to produce the rapeseed oil.

### Claim 14

A method of generating a genomically doubled Brassica napus rapeseed seed, the method comprising:
(a) applying a magnetic field to seeds of a euploid Brassica napus; and
(b) contacting said seeds of said euploid Brassica napus with a G2/M cell cycle inhibitor comprising a microtubule polymerization inhibitor under said magnetic field for targeted delivery of said microtubule polymerization inhibitor to chromatin fibers in nuclei of said seeds;
thereby generating the genomically doubled Brassica napus rapeseed seed.

### Claim 15 (depends on 14)

The method of claim 14, wherein said microtubule polymerization inhibitor is selected from the group consisting of colchicine, nocodazole, oryzaline, trifluraline and vinblastine sulphate.

### Claim 16 (depends on 10)

A tissue culture comprising the regenerable cell of claim 10.

### Claim 17 (depends on 1)

The plant of claim 1, wherein said microtubule polymerization inhibitor is selected from the group consisting of colchicine, nocodazole, oryzaline, trifluraline and vinblastine sulphate.

### Claim 18 (depends on 7)

The plant part of claim 7, wherein said seed is a hybrid seed.