# US 8,772,576 B2 · Herbicide-resistant inoculant strains

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- Number: US 8,772,576 B2
- Document ID: US8772576B2
- Status: Granted
- Kind: B2
- Application number: 13/393,869
- Filed: 2010-09-03
- Published: 2012-10-18
- Granted: 2014-07-08
- Earliest claimed: 2009-09-03
- Applicants: L. David Kuykendall
- Inventors: L. David Kuykendall
- Web page: https://patentgrep.com/patent/US8772576B2

## CPC

- C12N 1/205: Bacterial isolates
- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- C12N 1/20: Bacteria; Culture media therefor
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Exemplary embodiments are directed to a herbicide resistant N2-fixing microorganism and to a method for enhancing N2 fixation by the herbicide resistant rhizobia in symbiosis with herbicide resistant or tolerant leguminous plants treated with herbicide. At least one preferred embodiment comprises mutant rhizobia strains selected based on tolerance of the broad spectrum glyphosate weed control agent, originally Roundup®. Preferred embodiments can be expected to outcompete both indigenous soil rhizobia and/or commercial rhizobia that are not resistant to the herbicide.

## Claims

### Claim 1

A method for enhancing the growth of a plant, the method comprising the step of administering an effective quantity of a glyphosate resistant microorganism, of the genus Bradyrhizobium, deposited as ATCC accession number PTA-10253 to the plant or in the vicinity of the plant.

### Claim 2 (depends on 1)

The method of claim 1, wherein:
the step of administering the microorganism is achieved by at least one of the following:
applying the microorganism to the seeds of the plant;
applying the microorganism to the plant;
applying the microorganism to the locus of the plant root; and
applying the microorganism by in-furrow spray.

### Claim 3 (depends on 2)

The method of claim 2, further comprising the step of applying a glyphosate-containing herbicide to the plant.

### Claim 4 (depends on 1)

The method of claim 1, wherein the plant is a legume, resistant to glyphosate.

### Claim 5 (depends on 4)

The method of claim 4, wherein the legume is a soybean plant.