# US 8,772,574 B2 · Spinach hybrid RX 06691714 and parents thereof

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- Number: US 8,772,574 B2
- Document ID: US8772574B2
- Status: Granted
- Kind: B2
- Application number: 13/436,652
- Filed: 2012-03-30
- Published: 2013-08-01
- Granted: 2014-07-08
- Earliest claimed: 2012-02-01
- Assignees: Seminis Vegetable Seeds, Inc.
- Applicants: Bernardus Baerends
- Inventors: Bernardus Baerends
- Web page: https://patentgrep.com/patent/US8772574B2

## CPC

- A01H 6/028: {Spinacia oleracea [spinach]}
- A01H 5/12: Leaves
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The invention provides seed and plants of spinach hybrid RX 06691714 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of spinach hybrid RX 06691714 and the parent lines thereof, and to methods for producing a spinach plant produced by crossing such plants with themselves or with another spinach plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the leaf and gametes of such plants.

## Claims

### Claim 1

A spinach plant comprising at least a first set of the chromosomes of spinach line SMB66-1143, a sample of seed of said line having been deposited under ATCC Accession Number PTA-12486.

### Claim 2

A seed comprising at least a first set of the chromosomes of spinach line SMB66-1143, a sample of seed of said line having been deposited under ATCC Accession Number PTA-12486.

### Claim 3 (depends on 1)

The plant of claim 1, which is inbred.

### Claim 4 (depends on 1)

The plant of claim 1, which is hybrid.

### Claim 5 (depends on 2)

The seed of claim 2, which is inbred.

### Claim 6 (depends on 2)

The seed of claim 2, which is hybrid.

### Claim 7 (depends on 4)

The plant of claim 4, wherein the hybrid plant is spinach hybrid RX 06691714, a sample of seed of said hybrid RX 06691714 having been deposited under ATCC Accession Number PTA-12504.

### Claim 8 (depends on 6)

The seed of claim 6, defined as a seed of spinach hybrid RX 06691714, a sample of seed of said hybrid RX 06691714 having been deposited under ATCC Accession Number PTA-12504.

### Claim 9 (depends on 2)

The seed of claim 2, defined as a seed of line SMB66-1143.

### Claim 10 (depends on 1)

A plant part of the plant of claim 1.

### Claim 11 (depends on 10)

The plant part of claim 10, further defined as a fruit, a ovule, pollen, a leaf, or a cell.

### Claim 12 (depends on 7)

A spinach plant having all the physiological and morphological characteristics of the spinach plant of claim 7.

### Claim 13 (depends on 1)

A tissue culture of regenerable cells of the plant of claim 1.

### Claim 14 (depends on 13)

The tissue culture according to claim 13, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.

### Claim 15 (depends on 13)

A spinach plant regenerated from the tissue culture of claim 13.

### Claim 16 (depends on 1)

A method of vegetatively propagating the plant of claim 1 comprising the steps of:
(a) collecting tissue capable of being propagated from a plant according to claim 1;
(b) cultivating said tissue to obtain proliferated shoots; and
(c) rooting said proliferated shoots to obtain rooted plantlets.

### Claim 17 (depends on 16)

The method of claim 16, further comprising growing at least a first plant from said rooted plantlets.

### Claim 18

A method of introducing a desired trait into a spinach line comprising:
(a) crossing a plant of line SMB66-1143 with a second spinach plant that comprises a desired trait to produce F1 progeny, a sample of seed of said line having been deposited under ATCC Accession Number PTA-12486;
(b) selecting an F1 progeny that comprises the desired trait;
(c) backcrossing the selected F1 progeny with a plant of line SMB66-1143 to produce backcross progeny;
(d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of spinach line SMB66-1143; and
(e) repeating steps (c) and (d) three or more times to produce selected fourth or higher backcross progeny that comprise the desired trait.

### Claim 19 (depends on 18)

A spinach plant produced by the method of claim 18.

### Claim 20

A method of producing a plant comprising an added trait, the method comprising introducing a transgene conferring the trait into a plant of hybrid RX 06691714, or line SMB66-1143, a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-12504, and ATCC Accession Number PTA-12486, respectively.

### Claim 21 (depends on 20)

A plant produced by the method of claim 20.

### Claim 22 (depends on 1)

The plant of claim 1, comprising a transgene.

### Claim 23 (depends on 22)

The plant of claim 22, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

### Claim 24 (depends on 1)

The plant of claim 1, comprising a single locus conversion.

### Claim 25 (depends on 24)

The plant of claim 24, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

### Claim 26

A method for producing a seed of a plant derived from at least one of hybrid RX 06691714, or line SMB66-1143 comprising the steps of:
(a) crossing a spinach plant of hybrid RX 06691714, or line SMB66-1143 with itself or a second spinach plant; a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-12504, and ATCC Accession Number PTA-12486, respectively; and
(b) allowing seed of a hybrid RX 06691714, or line SMB66-1143-derived spinach plant to form.

### Claim 27 (depends on 26)

The method of claim 26, further comprising the steps of:
(c) selfing a plant grown from said hybrid RX 06691714, or SMB66-1143-derived spinach seed to yield additional hybrid RX 06691714, or line SMB66-1143-derived spinach seed;
(d) growing said additional hybrid RX 06691714, or line SMB66-1143-derived spinach seed of step (c) to yield additional hybrid RX 06691714, or line SMB66-1143-derived spinach plants; and
(e) repeating the crossing and growing steps of (c) and (d) to generate at least a first further hybrid RX 06691714, or line SMB66-1143-derived spinach plant.

### Claim 28 (depends on 26)

The method of claim 26, wherein the second spinach plant is of an inbred spinach line.

### Claim 29 (depends on 26)

The method of claim 26, comprising crossing line SMB66-1143 with line SSB 66-1129F, a sample of seed of said lines having been deposited under ATCC Accession Number PTA-12486, and ATCC Accession Number PTA-12019, respectively.

### Claim 30 (depends on 27)

The method of claim 27, further comprising:
(f) crossing the further hybrid RX 06691714, or SMB66-1143-derived spinach plant with a second spinach plant to produce seed of a hybrid progeny plant.

### Claim 31 (depends on 7)

A plant part of the plant of claim 7.

### Claim 32 (depends on 31)

The plant part of claim 31, further defined as a fruit, a ovule, pollen, a leaf, or a cell.

### Claim 33 (depends on 1)

A method of producing a spinach seed comprising crossing the plant of claim 1 with itself or a second spinach plant and allowing seed to form.

### Claim 34 (depends on 1)

A method of producing a spinach comprising:
(a) obtaining a plant according to claim 1, wherein the plant has been cultivated to maturity; and
(b) collecting a spinach from the plant.

### Claim 35

A method of producing a plant of spinach hybrid RX 06691714 comprising a single locus conversion, the method comprising crossing a plant of line SSB 66-1129F with a plant of line SMB66-1143, wherein at least one of said lines comprises a single locus conversion, a sample of seed of said lines SSB 66-1129F and SMB66-1143 having been deposited under ATCC Accession No. PTA-12019 and ATCC Accession Number PTA-12486, respectively.