# US 8,772,468 B2 · Splice variant specific messenger RNA transcripts as biomarkers of parkinson's disease

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- Number: US 8,772,468 B2
- Document ID: US8772468B2
- Status: Granted
- Kind: B2
- Application number: 13/240,821
- Filed: 2011-09-22
- Published: 2013-03-28
- Granted: 2014-07-08
- Earliest claimed: 2011-09-22
- Assignees: Rosalind Franklin University of Medicine and Science
- Applicants: Judith A. Potashkin
- Inventors: Judith A. Potashkin
- Web page: https://patentgrep.com/patent/US8772468B2

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- C12Q 2600/158: Expression markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention provides a method and a diagnostic kit for diagnosing the presence of Parkinson's disease in a human subject. The method includes the steps of: (1) extracting RNA molecules from a blood sample of the human subject to define a test sample; (2) measuring the amount of each RNA molecule having Sequence ID Nos. 1-14 in the test sample; (3) comparing the amount of each of the RNA molecules having Sequence ID Nos. 1-14 to the amount of RNA molecules having Sequence ID Nos. 1-14 present in a control sample to determine how many of the RNA molecules of Sequence ID Nos. 1-14 are present in a significant amount in the test sample greater or less than in the control sample to define a number of biomarkers; and (4) diagnosing the presence of Parkinson's disease in the human subject if the number of biomarkers is equal to or greater than five.

## Claims

### Claim 1

A kit for diagnosing the presence of Parkinson's disease in a human subject comprising:
14 containers each containing a forward and reverse primer pair to amplify one of each of a splice variant having Sequence ID Nos. 1-14 and power SYBR® dye; and
a set of instructions on the conditions for conducting real time PCR on a test sample and for diagnosing Parkinson's disease.

### Claim 2 (depends on 1)

The kit of claim 1 wherein the test sample comprises RNA molecules extracted from a whole blood sample of the human subject.