# US 8,772,466 B2 · Zea mays NFB2 promoter

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- Number: US 8,772,466 B2
- Document ID: US8772466B2
- Status: Granted
- Kind: B2
- Application number: 12/331,214
- Filed: 2008-12-09
- Published: 2009-05-28
- Granted: 2014-07-08
- Earliest claimed: 2005-12-07
- Assignees: Monsanto Technology LLC
- Applicants: Donald E. Nelson; Brendan S. Hinchey; Michelle Lacy; James A. Morrell; Andrey A. Boukharov; Zhaolong Li; Wei Wu; Linda Lutfiyya; Maolong Lu; David K. Kovalic; Qi Wang; Ping Li; Yongwei Cao
- Inventors: Donald E. Nelson; Brendan S. Hinchey; Michelle Lacy; James A. Morrell; Andrey A. Boukharov; Zhaolong Li; Wei Wu; Linda Lutfiyya; Maolong Lu; David K. Kovalic; Qi Wang; Ping Li; Yongwei Cao
- Web page: https://patentgrep.com/patent/US8772466B2

## CPC

- C12N 15/8222: {Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation}
- C12N 15/8223: {Vegetative tissue-specific promoters}
- C12N 15/823: {Reproductive tissue-specific promoters}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides non-coding regulatory element polynucleotide molecules isolated from the transcription factor Nuclear Factor B (NFB2) gene of Zea mays and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Zea mays NFB2 regulatory polynucleotide sequences, and methods for preparing and using the same.

## Claims

### Claim 1

A polynucleotide molecule comprising a nucleic acid sequence selected from the group consisting of:
(a) the nucleic acid sequence of SEQ ID NO:1 or SEQ ID NO:2; and
(b) a fragment of at least 50 contiguous bases of SEQ ID NO:1 or SEQ ID NO:2 wherein said fragment comprises promoter activity, and wherein the polynucleotide molecule is operably linked to a heterologous transcribable polynucleotide molecule.

### Claim 2 (depends on 1)

The polynucleotide molecule of claim 1, wherein said transcribable polynucleotide molecule comprises a coding sequence of agronomic interest.

### Claim 3 (depends on 1)

The polynucleotide molecule of claim 1, wherein said transcribable polynucleotide molecule comprises a coding sequence controlling the phenotype of a trait selected from the group consisting of: herbicide tolerance, insect control, modified yield, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, plant growth and development, starch production, modified oils production, high oil production, modified fatty acid content, high protein production, fruit ripening, enhanced animal and human nutrition, biopolymers, environmental stress resistance, pharmaceutical peptides and secretable peptides, improved processing traits, improved digestibility, enzyme production, flavor, nitrogen fixation, hybrid seed production, fiber production, and biofuel production.

### Claim 4 (depends on 3)

The polynucleotide molecule of claim 3, wherein said herbicide tolerance coding sequence is selected from the group consisting of coding sequences for: phosphinothricin acetyltransferase, glyphosate resistant EPSPS, hydroxyphenyl pyruvate dehydrogenase, dalapon dehalogenase, bromoxynil resistant nitrilase, anthranilate synthase, glyphosate oxidoreductase and glyphosate-N-acetyl transferase.

### Claim 5 (depends on 1)

A transgenic plant cell stably transformed with the polynucleotide molecule of claim 1.

### Claim 6 (depends on 1)

A transgenic plant stably transformed with the polynucleotide molecule of claim 1.

### Claim 7 (depends on 6)

A seed of said transgenic plant of claim 6, wherein the seed comprises the polynucleotide molecule.

### Claim 8 (depends on 6)

A progeny of the plant of claim 6, wherein the progeny comprises the polynucleotide molecule.

### Claim 9 (depends on 5)

The transgenic plant cell of claim 5, wherein said plant cell is from a monocotyledonous plant selected from the group consisting of wheat, maize, rye, rice, corn, oat, barley, turfgrass, sorghum, millet and sugarcane.

### Claim 10 (depends on 6)

The transgenic plant of claim 6, wherein said plant is a monocotyledonous plant selected from the group consisting of wheat, maize, rye, rice, corn, oat, barley, turfgrass, sorghum, millet and sugarcane.

### Claim 11 (depends on 10)

A seed of the transgenic plant of claim 10, wherein the seed comprises the polynucleotide molecule.

### Claim 12 (depends on 5)

A transgenic plant cell of claim 5, wherein said plant cell is from a dicotyledonous plant selected from the group consisting of tobacco, tomato, potato, soybean, cotton, canola, sunflower and alfalfa.

### Claim 13 (depends on 6)

The transgenic plant of claim 6, wherein said plant is a dicotyledonous plant selected from the group consisting of tobacco, tomato, potato, soybean, cotton, canola, sunflower and alfalfa.

### Claim 14 (depends on 13)

A seed of the transgenic plant of claim 13, wherein the seed comprises the polynucleotide molecule.

### Claim 15 (depends on 1)

A method of inhibiting weed growth in a field of transgenic glyphosate-tolerant crop plants comprising: planting transgenic plants transformed with an expression cassette comprising the polynucleotide molecule of claim 1 wherein the transcribable polynucleotide molecule confers glyphosate tolerance to said transgenic plants; and applying glyphosate to the field at an application rate that inhibits the growth of weeds.