# US 8,772,465 B2 · IRES functioning in plant

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- Number: US 8,772,465 B2
- Document ID: US8772465B2
- Status: Granted
- Kind: B2
- Application number: 10/586,052
- Filed: 2005-01-13
- Published: 2008-03-06
- Granted: 2014-07-08
- Earliest claimed: 2004-01-15
- Assignees: Riken
- Applicants: Minami Matsui; Yoshiharu Yamamoto; Kazuhito Gohda; Kumiko Suzuki
- Inventors: Minami Matsui; Yoshiharu Yamamoto; Kazuhito Gohda; Kumiko Suzuki
- Web page: https://patentgrep.com/patent/US8772465B2

## CPC

- C12N 15/8216: {Methods for controlling, regulating or enhancing expression of transgenes in plant cells}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

It is intended to provide for a polycistronic expression pattern in a plant used as a host. The present invention provides a polynucleotide which comprises the following DNA (a) or (b) and functions as an IRES (internal ribosome entry site) in a plant: (a) DNA of the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4; or (b) DNA of a nucleotide sequence derived from the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4 by the substitution, deletion, addition, and insertion of one or more bases and having a function of positively regulating the translation of a nucleic acid located downstream thereof.

## Claims

### Claim 1

An isolated polynucleotide which functions as an IRES (internal ribosome entry site) in a plant and comprises two to fifteen repeats of the nucleotide sequence represented by SEQ ID NO: 1 without a spacer sequence.

### Claim 2 (depends on 1)

The polynucleotide according to claim 1, wherein the polynucleotide further comprises at least a coding region and/or a promoter.

### Claim 3 (depends on 1)

A vector comprising the polynucleotide according to claim 1.

### Claim 4 (depends on 1)

A transformant transformed with the polynucleotide according to claim 1.

### Claim 5 (depends on 1)

A transgenic plant having the polynucleotide according to claim 1 incorporated in the genome.

### Claim 6 (depends on 1)

A method of regulating gene expression in a plant, said method comprising:
constructing the polynucleotide according to claim 1; and
transforming the polynucleotide into a plant host,
wherein the translation of a gene located downstream of the repeats of the nucleotide sequence represented by SEQ ID NO: 1 is positively regulated in the transformed host.

### Claim 7 (depends on 3)

A transformant transformed with the vector according to claim 3.

### Claim 8 (depends on 3)

A method of regulating gene expression in a plant, said method comprising:
constructing the vector according to claim 3; and
transforming the vector into a plant host,
wherein the translation of a gene located downstream of the repeats of the nucleotide sequence represented by SEQ ID NO: 1 is positively regulated in the transformed host.