# US 8,772,228 B2 · Aligned nanofibers and related methods of use

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,772,228 B2
- Document ID: US8772228B2
- Status: Granted
- Kind: B2
- Application number: 12/031,250
- Filed: 2008-02-14
- Published: 2008-12-04
- Granted: 2014-07-08
- Earliest claimed: 2007-02-14
- Assignees: Northwestern University
- Applicants: Samuel I. Stupp; Shuming Zhang; Alvaro Mata; Megan A Greenfield
- Inventors: Samuel I. Stupp; Shuming Zhang; Alvaro Mata; Megan A Greenfield
- Web page: https://patentgrep.com/patent/US8772228B2

## CPC

- C12N 5/0662: {Stem cells}
- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C12N 2533/50: Proteins
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to nanofibers. In particular, the present invention provides aligned nanofiber bundle assemblies. In some embodiments, the aligned nanofiber bundle assemblies are used for tissue regeneration, controlled growth of cells, and related methods (e.g., diagnostic methods, research methods, drug screening).

## Claims

### Claim 1

A composition comprising a bundle of a plurality of longitudinally aligned nanofibers, wherein said nanofibers comprise self-assembling peptide amphiphile molecules, wherein said self-assembling peptide amphiphile molecules comprise a hydrophobic tail, a peptide segment configured for beta-sheet formation, and a charged segment; wherein said self-assembling peptide amphiphile molecules interact to form aligned nanofibers; and wherein said bundle of aligned nanofibers comprises 15-35 nanofibers.

### Claim 2 (depends on 1)

The composition of claim 1, wherein said hydrophobic tail comprises an alkyl tail.

### Claim 3 (depends on 2)

The composition of claim 2, wherein said alkyl tail comprises at least 16 carbon atoms.

### Claim 4 (depends on 1)

The composition of claim 1, wherein said peptide segment configured for beta-sheet formation comprises uncharged amino acid residues.

### Claim 5 (depends on 4)

The composition of claim 4, wherein said peptide segment configured for beta-sheet formation comprises three alanine and three valine amino acid residues.

### Claim 6 (depends on 1)

The composition of claim 1, wherein said charged segment comprises a charged peptide segment comprising charged amino acid residues.

### Claim 7 (depends on 6)

The composition of claim 6, wherein said charged amino acid residues comprises three glutamic acid residues.

### Claim 8

A bundle of longitudinally aligned nanofibers, wherein said nanofibers comprise self-assembling peptide amphiphile molecules, wherein said self-assembling peptide amphiphile molecules comprise a hydrophobic tail, a peptide segment and a charged segment; wherein said bundle of nanofibers comprises 15-35 nanofibers.