# US 8,772,036 B2 · Automated method for sample distribution and isolation of an analyte with a solid support

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- Number: US 8,772,036 B2
- Document ID: US8772036B2
- Status: Granted
- Kind: B2
- Application number: 13/169,559
- Filed: 2011-06-27
- Published: 2012-06-28
- Granted: 2014-07-08
- Earliest claimed: 2010-06-29
- Assignees: Roche Molecular Systems, Inc.
- Applicants: Paul Frank; Andreas Gisler; Robert Huesler; Rolf Knobel; Siegfried Mueller; Urs Schnieper; Heinz Trueeb
- Inventors: Paul Frank; Andreas Gisler; Robert Huesler; Rolf Knobel; Siegfried Mueller; Urs Schnieper; Heinz Trueeb
- Web page: https://patentgrep.com/patent/US8772036B2

## CPC

- G01N 35/1095: {for supplying the samples to flow-through analysers (for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00)}
- G01N 35/0098: {involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C)}
- G01N 35/028: {having reaction cells in the form of microtitration plates}
- G01N 35/1067: {for transfer to or from containers having different spacing}
- G01N 35/1074: {arranged in a two-dimensional array}
- B01L 3/0275: {Interchangeable or disposable dispensing tips}
- G01N 2035/00326: {Analysers with modular structure}
- G01N 2035/103: {using disposable tips}
- G01N 2035/1069: {by adjusting the spacing between multiple probes of a single transferring head}
- Y10T 436/11: Automated chemical analysis
- Y10T 436/114998: with treatment or replacement of aspirator element [e.g., cleaning, etc.]
- Y10T 436/119163: with aspirator of claimed structure
- Y10T 436/2575: Volumetric liquid transfer
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A method is described for distributing samples within an automated analyzer from a linear arrangement of sample vessels to a processing plate in a two-dimensional n×m arrangement wherein samples are sorted, followed by transfer with a pipetting device with a linear arrangement to a processing vessel in a two-dimensional n×m arrangement and subsequent processing of samples using a second pipetting device which has a two-dimensional n×m arrangement.

## Claims

### Claim 1

A method for isolating analytes that may be present in fluid samples in an automated system, the method comprising the automated steps of:
a) loading samples on the automated system with identifiers, or identifying loaded samples according to sample type and analytical test to be performed;
b) transferring instructions from a control unit to a first and a second pipetting device comprising allocation of individual samples to a two-dimensional n×m arrangement of individual tests in a processing plate;
c) transferring said fluid samples from at least two sample vessels to a at least two vessels of said processing plate with a first pipetting device comprising at least two pipetting units in linear arrangement, wherein said pipetting units are coupled to pipette tips;
d) combining together a solid support material and said fluid sample in a well of said processing plate for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;
e) isolating the solid support material from other material present in the fluid sample in a separation station; and
f) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the materials one or more times with a wash buffer, wherein said solid support material and said washing buffer are mixed by aspirating and dispensing with a second pipetting device, said second pipetting device comprising pipetting units in a two-dimensional n×m arrangement for coupling to pipette tips;
wherein said pipette tips used in step c) are re-used in step f).

### Claim 2 (depends on 1)

The method of claim 1, wherein said wells of said processing plate are integrally formed.

### Claim 3 (depends on 1)

The method of claim 1, wherein said processing plate is a multiwell plate with a two-dimensional n×m arrangement of vessels.

### Claim 4 (depends on 1)

The method of claim 1, wherein said pipette tips are replaced in a tip rack in a two-dimensional n×m arrangement following step c).

### Claim 5 (depends on 1)

The method of claim 1, comprising the step of assigning any one pipette tip in said pipette tip rack to a specific vessel of said processing plate with a two-dimensional n×m arrangement of vessels.

### Claim 6 (depends on 1)

The method of claim 1, wherein the distance between two adjacent sample vessels is different from the distance between two adjacent vessels of the processing plate, and wherein the distance between two adjacent pipetting units of the first pipetting device is adjustable.

### Claim 7 (depends on 1)

The method of claim 1, wherein in step b) instructions are transferred from said control unit to a first processor for transferring samples with said first pipetting device, and instructions are transferred from said control unit to a second processor for the steps carried out by said second pipetting device.