Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
AssigneeBayer Pharmaceuticals Corporation
InventorsSham-Yuen Chan, Hsinyi Yvette Tang, Yiwen Tao, Yongjian Wu, Ruth Kelly
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for increased production of a secreted, recombinant protein product through the introduction of molecular chaperones in a mammalian host cell. The present invention also relates to a mammalian host cell with enhanced expression of a secreted recombinant protein product by coexpressing at least one chaperone protein.
Claims8
A mammalian host cell for enhanced expression of a recombinant protein product, said mammalian cell having genetic material coding for expression of said recombinant protein product and transformed with at least one expression vector comprising DNA encoding calreticulin as a chaperone protein, wherein the recombinant protein product is bikunin or fragment thereof.
A mammalian host cell for enhanced expression of a recombinant protein product, said mammalian cell having genetic material coding for expression of said recombinant protein product and transformed with at least one expression vector comprising DNA encoding calreticulin as a chaperone protein, wherein the recombinant protein product is Factor VIII or fragment thereof.
Dependencies are inferred from the claim text.