# US 8,772,021 B2 · Expression vector for animal cell comprising at least one copy of MAR DNA sequences at the 3′ terminal of transcription termination region of a gene and method for the expression of foreign gene using the vector

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- Number: US 8,772,021 B2
- Document ID: US8772021B2
- Status: Granted
- Kind: B2
- Application number: 11/817,756
- Filed: 2006-03-04
- Published: 2008-05-01
- Granted: 2014-07-08
- Earliest claimed: 2005-03-04
- Assignees: Celltrion Inc.
- Applicants: Jong-mook Kim; Hye-jin Hong; Hyun-joo Lee; Moon-kyoung So; Soo-young Lee; Jong-moon Cho; Bok-hwan Chun; Seung-suh Hong; Myung-sam Cho
- Inventors: Jong-mook Kim; Hye-jin Hong; Hyun-joo Lee; Moon-kyoung So; Soo-young Lee; Jong-moon Cho; Bok-hwan Chun; Seung-suh Hong; Myung-sam Cho
- Web page: https://patentgrep.com/patent/US8772021B2

## CPC

- C12N 15/85: for animal cells
- C12N 2830/42: being an intron or intervening sequence for splicing and/or stability of RNA
- C12N 2830/46: elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
- C12N 2840/20: translation of more than one cistron
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided are an expression vector for an animal cell including a promoter, a cloning site or a polynucleotide encoding foreign product, and a transcription terminator, all of which are operably connected each other within the expression vector, in which at least one copy of human β-globin MAR sequence is attached to the 31 terminal of the transcription terminator, and a method of expressing a foreign gene using the expression vector.

## Claims

### Claim 1

An expression vector for an animal cell comprising
a promoter,
a cloning site or a polynucleotide encoding a foreign gene product, and
a transcription terminator,
all of which are operably connected to each other within the expression vector, wherein the vector comprises a polynucleotide containing nucleotides 4161 to 10134 of SEQ ID NO:2, in which two copies of human β-globulin MAR sequences are connected adjacently to each other, wherein said two copies of human β-globin matrix attachment region (MAR) are located at the 3′ terminal of the transcription terminator.

### Claim 2 (depends on 1)

The expression vector of claim 1, wherein the promoter is SV40 early promoter or CMV promoter.

### Claim 3 (depends on 1)

The expression vector of claim 1, wherein the polynucleotide encoding the foreign product is a gene encoding lacZ, immunoglobulin, granulocyte colony-stimulating factor (GCSF) or erythropoietin (EPO).

### Claim 4 (depends on 1)

The expression vector of claim 1, wherein the transcription terminator is SV40 virus transcription terminator.

### Claim 5 (depends on 1)

The expression vector of claim 1, wherein the expression vector is SEQ ID NO: 2 or a pCMVMM-IgG expression vector having the vector map shown in FIG. 14.

### Claim 6 (depends on 1)

A method of expressing a foreign gene, comprising transfecting an animal cell with the expression vector according to claim 1 which comprises a polynucleotide encoding the foreign gene product, and culturing the transfected animal cell.

### Claim 7 (depends on 6)

The method of claim 6, wherein the animal cell is selected from the group consisting of Chinese hamster ovary (CHO) cells, Baby hamster kidney (BHK) cells, NS0 cells and human cells.