# US 8,772,003 B2 · Bacterial cellulose film and uses thereof

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- Number: US 8,772,003 B2
- Document ID: US8772003B2
- Status: Granted
- Kind: B2
- Application number: 13/114,014
- Filed: 2011-05-23
- Published: 2011-11-24
- Granted: 2014-07-08
- Earliest claimed: 2010-05-24
- Assignees: Nympheas International Biomaterial Corp.
- Applicants: Yi-Chuan Lin; Yuh-Chyun Wey; Mei-Ling Lee
- Inventors: Yi-Chuan Lin; Yuh-Chyun Wey; Mei-Ling Lee
- Web page: https://patentgrep.com/patent/US8772003B2

## CPC

- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- A61P 17/00: Drugs for dermatological disorders
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

A bacterial cellulose film is provided, including microbial fibers consisting of bacterial cellulose, wherein the microbial fibers are arranged in a density gradient in a direction of the bacterial cellulose film thickness.

## Claims

### Claim 1

A method, for producing a bacterial cellulose film, comprises a static fermentation step of Gluconacetobacter, wherein the static fermentation step comprises two-step culture temperature of 25˜28° C. at the first step and 29˜30° C. at the second and starts from initial concentration of Gluconacetobacter in a range of 102-105/ml, and wherein the bacterial cellulose film comprises microbial fibers consisting of bacterial cellulose and the microbial fibers are arranged in a density gradient in a direction of the bacterial cellulose film thickness.