# US 8,772,001 B2 · Alcohol product processes > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,772,001 B2 - Document ID: US8772001B2 - Status: Granted - Kind: B2 - Application number: 12/138,681 - Filed: 2008-06-13 - Published: 2009-01-15 - Granted: 2014-07-08 - Earliest claimed: 2003-03-10 - Assignees: Novozymes A/S - Applicants: Hans Sejr Olsen; Sven Pedersen; Rikke Monica Festersen - Inventors: Hans Sejr Olsen; Sven Pedersen; Rikke Monica Festersen - Web page: https://patentgrep.com/patent/US8772001B2 ## CPC - C12P 7/06: Ethanol, i.e. non-beverage - C12C 5/004: {Enzymes} - C12C 7/04: Preparation or treatment of the mash - C12N 9/2411: {Amylases} - C12N 9/2414: {Alpha-amylase (3.2.1.1.)} - C12N 9/2417: {from microbiological source} - C12N 9/242: {Fungal source} - C12N 9/2425: {Beta-amylase (3.2.1.2)} - C12N 9/2428: {Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase} - C12P 7/10: substrate containing cellulosic material - C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60) - C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} - C12Y 302/01001: Alpha-amylase (3.2.1.1) - C12Y 302/01003: Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase - Y02E 50/10: Biofuels, e.g. bio-diesel - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12C: BEER; PREPARATION OF BEER BY FERMENTATION (ageing or ripening by storing C12H1/22; methods for reducing the alcohol content after fermentation C12H3/00; methods for increasing the alcohol content after fermentation C12H6/00; venting devices for casks, barrels or the like C12L9/00); PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES - Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION ## Abstract Processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of the granular starch followed by simultaneous saccharification and fermentation, and optionally recovery of ethanol, are described. ## Claims ### Claim 1 A process for production of an alcohol product comprising the sequential steps of: (a) holding a slurry comprising water and granular starch in the presence of an acid alpha-amylase and a glucoamylase at a temperature of 0° C. to 20° C. below the initial gelatinization temperature of the granular starch for a period between 5 minutes and 12 hours, and (b) fermenting the slurry in the presence of an acid alpha-amylase, a glucoamylase and a yeast at a temperature between 10° C. and 35° C. to produce the alcohol product, wherein step (a) is performed in the presence of a cellulase and a xylanase. ### Claim 2 (depends on 1) The process of claim 1, further comprising recovering the alcohol product. ### Claim 3 (depends on 1) The process of claim 1, wherein the alcohol product is fuel ethanol, potable ethanol and/or industrial ethanol. ### Claim 4 (depends on 1) The process of claim 1, wherein the temperature during step (b) is between 29° C. and 35° C. ### Claim 5 (depends on 1) The process of claim 1, wherein the pH during step (a) is in the range of 3-7. ### Claim 6 (depends on 1) The process of claim 1, wherein the pH during step (b) is in the range of 3-7. ### Claim 7 (depends on 1) The process of claim 1, wherein the starch slurry has 5-60% DS granular starch. ### Claim 8 (depends on 1) The process of claim 1, wherein the granular starch is obtained from tubers, roots, stems, fruits, seeds or whole grain. ### Claim 9 (depends on 1) The process of claim 1, wherein the granular starch is obtained from corn, cobs, wheat, barley, rye, milo, sago, cassaya, manioc, tapioca, sorghum, rice or potatoes. ### Claim 10 (depends on 1) The process of claim 1, wherein the granular starch is obtained from cereals. ### Claim 11 (depends on 1) The process of claim 1, wherein the granular starch is obtained from dry milling or wet milling of whole grain. ### Claim 12 (depends on 1) The process of claim 1, wherein the acid alpha-amylase and the glucoamylase are present in step (a) in a ratio of between 0.3 and 5.0 Acid Fungal Alpha-amylase Units/AmyloGlucosidase Units (AFAU/AGU). ### Claim 13 (depends on 1) The process of claim 1, wherein the acid alpha-amylase and the glucoamylase are present in step (b) in a ratio of between 0.3 and 5.0 Acid Fungal Alpha-amylase Units/AmyloGlucosidase Units (AFAU/AGU). ### Claim 14 (depends on 1) The process of claim 1, wherein the acid alpha-amylase is an acid fungal alpha-amylase. ### Claim 15 (depends on 14) The process of claim 14, wherein the acid fungal alpha-amylase is obtained from a strain of Aspergillus. ### Claim 16 (depends on 1) The process of claim 1, wherein the acid alpha-amylase is an acid bacterial alpha-amylase. ### Claim 17 (depends on 16) The process of claim 16, wherein the acid bacterial alpha-amylase is derived from a strain of B. amyloliquefaciens, B. licheniformis, or B. stearothermophilus. ### Claim 18 (depends on 1) The process of claim 1, wherein the glucoamylase is obtained from a strain of Aspergillus, Clostridium, or Talaromyces. ### Claim 19 (depends on 1) The process of claim 1, wherein the glucoamylase is obtained from a strain of Aspergillus niger. ### Claim 20 (depends on 1) The process of claim 1, further comprising performing step (a) in the presence of a phytase. ### Claim 21 (depends on 1) The process of claim 1, further comprising performing step (b) in the presence of phytase. ### Claim 22 (depends on 1) The process of claim 1, further comprising performing steps (a) and (b) in the presence of a phytase. ### Claim 23 (depends on 1) The process of claim 1, wherein step (b) is performed in the presence of a cellulase and a xylanase. ### Claim 24 (depends on 1) The process of claim 1, wherein the period in step (a) is between 5 minutes and 1¼ hours. ### Claim 25 (depends on 1) The process of claim 1, wherein the period in step (a) is between 5 minutes and 1 hour. ### Claim 26 (depends on 1) The process of claim 1, wherein the period in step (a) is between 40 minutes and 1 hour. ### Claim 27 (depends on 1) The process of claim 1, wherein the period in step (a) is between 50 minutes and 1 hour. ### Claim 28 (depends on 1) The process of claim 1, wherein the fermentation in step (b) is for a period between 20 and 250 hours. ### Claim 29 (depends on 1) The process of claim 1, wherein the temperature during step (a) is 45 to 62° C. ### Claim 30 (depends on 1) The process of claim 1, further comprising performing step (b) in the presence of a protease.