Transformant for enhancing bioethanol production, and method for producing ethanol by using said strain
AssigneeKorea University Research and Business Foundation
InventorsSung Ok Han, Kyung Ok Yu, Seung Wook Kim
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Abstract
The present invention relates to a transformant for inhibiting glycerol production through deletion of glycerol producing genes of Saccharomyces cerevisiae modified so as to use glycerol as a fermentation source, or enhancing bioethanol production through overexpression of TATA-binding proteins, SPT3 and SPT15, and a method for producing ethanol by using the transformant.
Claims4
A genetically engineered yeast cell, wherein said cell comprises:
- a disruption of a gene encoding glycerol-3-phosphate dehydrogenase 2 and a disruption of a FPS1 (glycerol facilitator channel) gene encoding yeast glycerol channel FPS1, wherein said cell further comprises a gene encoding glycerol dehydrogenase, a gene encoding dihydroxyacetone kinase, and a gene encoding glycerol uptake protein, wherein said cell comprises a gene encoding Saccharomyces cerevisiae pyruvate decarboxylase and a gene encoding alcohol dehydrogenase, wherein said cell is Saccharomyces cerevisiae YPH499fps1Δgpd2Δ (pGcyaDakAdhPdc, pGupCas), wherein the accession number of Saccharomyces cerevisiae YPH499fps1Δgpd2Δ (pGcyaDakAdhPdc, pGupCas) is KCCM11152P.
A genetically engineered yeast cell, wherein the cell is Saccharomyces cerevisiae YPH499fps1Δgpd2Δ (pGcyaDak, pGup1Cas), wherein the accession number of Saccharomyces cerevisiae YPH499fps1Δgpd2Δ (pGcyaDak, pGup1 Cas) is KCCM11071P.
Dependencies are inferred from the claim text.