# US 8,771,999 B2 · Low energy, high substrate efficiency, anaerobic, deep, bubble column fermentation processes > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,771,999 B2 - Document ID: US8771999B2 - Status: Granted - Kind: B2 - Application number: 13/243,062 - Filed: 2011-09-23 - Published: 2013-03-28 - Granted: 2014-07-08 - Earliest claimed: 2011-09-23 - Assignees: Coskata, Inc. - Applicants: Robert Hickey; Richard E. Tobey - Inventors: Robert Hickey; Richard E. Tobey - Web page: https://patentgrep.com/patent/US8771999B2 ## CPC - C12P 7/00: Preparation of oxygen-containing organic compounds - C12P 7/06: Ethanol, i.e. non-beverage - C12P 7/54: Acetic acid (vinegar C12J) - Y02E 50/10: Biofuels, e.g. bio-diesel - Y02P 20/582: Recycling of unreacted starting or intermediate materials - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract Bioconversion processes are disclosed that enable high conversion efficiencies of gas substrate containing both carbon monoxide and hydrogen to oxygenated organic compounds via the carbon monoxide and hydrogen pathways using anaerobic, deep, bubble column fermentation in a cost effective manner. The high conversion efficiency processes of this invention comprise the combination of using at least two deep, bubble column reactors in flow series and using certain feed gas compositions and microbubbles while avoiding carbon monoxide inhibition. ## Claims ### Claim 1 A process for the bioconversion of a gas feed containing gas substrate comprising carbon monoxide, carbon dioxide and hydrogen in an aqueous menstruum containing microorganisms suitable for converting said gas substrate to oxygenated organic compound comprising: a. providing the aqueous menstruum in at least one primary deep, bubble column reactor to a height of at least 10 meters wherein the gas feed is injected in a bottom portion of the reactor and wherein the pressure of the aqueous menstruum is at least about 100 kPa gauge at said portion, b. continuously supplying as microbubbles said gas feed by injection using a motive liquid to the bottom portion of said reactor, said gas feed: (i) containing at least about 2 mole percent nitrogen, (ii) containing at least about 5 mole percent carbon dioxide, (iii) having a mole ratio of hydrogen to carbon monoxide between about 1:6 to 6:1, and (iv) having mole fractions of carbon dioxide and nitrogen sufficient to provide a carbon monoxide partial pressure at the point of injection of less than about 80 kPa; c. converting less than about 80 percent of the carbon monoxide in the gas feed supplied to the primary deep, bubble column reactor to oxygenated organic compound and providing an off-gas above the aqueous menstruum; d. optionally removing at least a portion of the carbon dioxide from the aqueous menstruum in the primary deep, bubble column reactor or from at least a portion of the off-gas from the primary deep, bubble column reactor; e. continuously supplying as microbubbles at least a portion of the off-gas by injection using a motive liquid to a lower portion of at least one sequential deep, bubble column reactor containing aqueous menstruum in which said off-gas passes upwardly through the aqueous menstruum wherein the off-gas has a mole fraction of nitrogen sufficient to avoid a mass transfer of carbon monoxide to the aqueous menstruum that results in carbon monoxide inhibition; and f. converting sufficient hydrogen and carbon monoxide in the off-gas to oxygenated organic compound such that at least about 75 percent of the total moles of carbon monoxide and hydrogen in the gas feed to the primary deep, bubble column reactor is converted to oxygenated compound. ### Claim 2 (depends on 1) The process of claim 1 wherein the oxygenated compound comprises at least one of acetic acid and ethanol. ### Claim 3 (depends on 2) The process of claim 2 wherein the gas feed to the primary deep, bubble column reactor has a mole ratio of hydrogen to carbon monoxide between about 1:4 to 2:1. ### Claim 4 (depends on 2) The process of claim 2 wherein the mole ratio of nitrogen to carbon dioxide in the gas substrate is within the range of 0.2:1 to 2:1. ### Claim 5 (depends on 4) The process of claim 4 wherein the carbon monoxide partial pressure at the location where the gas substrate enters the primary deep, bubble column reactor is between about 20 and 75 kPa. ### Claim 6 (depends on 1) The process of claim 1 wherein in step (c) about 60 to 75 percent of the carbon monoxide in the gas feed supplied to the primary deep, bubble column reactor is converted to oxygenated organic compound. ### Claim 7 (depends on 1) The process of claim 1 wherein at least a portion of the carbon dioxide is removed from at least one of the aqueous menstruum in the primary, deep, bubble column reactor and the off-gas from the primary, deep, bubble column reactor. ### Claim 8 (depends on 1) The process of claim 1 wherein the pressure at the location where the gas feed enters the deep, bubble column reactor is at a pressure in the range of about 150 to 300 kPa gauge. ### Claim 9 (depends on 1) The process of claim 1 wherein the gas feed to the sequential reactor contains between 15 and 50 mole percent of total hydrogen and carbon monoxide, between about 5 and 35 mole percent carbon dioxide and between about 15 to 50, mole percent nitrogen. ### Claim 10 (depends on 1) The process of claim 1 wherein gas substrate is fed to more than one primary deep, bubble column reactor and the off-gas from each is combined and treated to remove at least a portion of the carbon dioxide to provide a combined treated off-gas and the combined treated off-gas is fed to one or more sequential reactors. ### Claim 11 (depends on 1) The process of claim 1 wherein at least about 90 percent (excluding any carbon dioxide removed) of the off-gas from the primary deep, bubble column reactor is supplied to the sequential deep, bubble column reactor. ### Claim 12 (depends on 1) The process of claim 1 wherein changing the size of the microbubbles is used to modulate the transfer of carbon monoxide to the aqueous menstruum in the primary deep, bubble column reactor. ### Claim 13 (depends on 12) The process of claim 12 wherein the injection is by a slot injector and the motive liquid comprises aqueous menstruum. ### Claim 14 (depends on 13) The process of claim 13 wherein the size of the microbubbles is changed to modulate the transfer of carbon monoxide to the aqueous menstruum in the sequential deep, bubble column reactor. ### Claim 15 (depends on 14) The process of claim 14 wherein the injection is by a slot injector and the motive liquid comprises aqueous menstruum. ### Claim 16 (depends on 1) The process of claim 1 wherein each of the primary deep, bubble column reactor and the sequential deep, bubble column reactor have a height of aqueous menstruum therein of between about 15 and 30 meters. ### Claim 17 (depends on 16) The process of claim 16 wherein the primary deep, bubble column reactor has a capacity of between about 5 and 25 million liters. ### Claim 18 (depends on 16) The process of claim 16 wherein more than one primary deep, bubble column reactors are used and the primary deep, bubble column reactor and the sequential deep, bubble column reactor. ### Claim 19 A process for the bioconversion of a gas feed containing gas substrate comprising carbon monoxide, carbon dioxide and hydrogen in an aqueous menstruum containing microorganisms suitable for converting said gas substrate to oxygenated organic compound comprising at least one of ethanol and acetic acid or salts thereof comprising: a. providing the aqueous menstruum in at least one primary deep, bubble column reactor to a height of at least 15 meters wherein the gas feed is injected in a bottom portion of the reactor, b. continuously supplying said gas feed by injection using a motive liquid comprising aqueous menstruum to the bottom portion of said reactor to form microbubbles in the range of between about 20 and 300 microns, said gas feed: (i) containing at least about 2 mole percent nitrogen, (ii) containing at least about 5 mole percent carbon dioxide, (iii) having a mole ratio of hydrogen to carbon monoxide between about 1:2 to 2:1, and (iv) having mole fractions of carbon dioxide and nitrogen sufficient to provide a carbon monoxide partial pressure at the point of injection of between 20 and 75 kPa; c. converting between about 60 and 75 percent of the carbon monoxide in the gas feed supplied to the primary deep, bubble column reactor to oxygenated organic compound and providing an off-gas above the aqueous menstruum, wherein the rate of flow of the motive liquid provides microbubbles of the gas feed of a size sufficient to provide a rate of transfer of carbon monoxide to the aqueous menstruum to achieve the about 60 to 75 percent conversion of carbon monoxide in the gas feed without building-up the concentration of dissolved carbon monoxide in the aqueous menstruum; d. continuously supplying a gas feed containing at least a portion of the off-gas from the primary deep, bubble column reactor to a lower portion of at least one sequential deep, bubble column reactor containing aqueous menstruum in which said off-gas passes upwardly through the aqueous menstruum wherein (i) the gas feed is supplied by injection using a motive liquid comprising aqueous menstruum to provide microbubbles of the gas feed and (ii) the off-gas has a mole fraction of nitrogen sufficient to avoid a mass transfer of carbon monoxide to the aqueous menstruum that results in carbon monoxide inhibition; and e. converting sufficient hydrogen and carbon monoxide in the off-gas to oxygenated organic compound in the sequential deep, bubble column reactor such that at least about 80 percent of the total moles of carbon monoxide and hydrogen in the gas feed to the primary deep, bubble column reactor is converted to oxygenated compound. ### Claim 20 (depends on 19) The process of claim 19 wherein at least a portion of the carbon dioxide is removed from the aqueous menstruum in the primary deep, bubble column reactor or from at least a portion of the off-gas from the primary deep, bubble column reactor.