# US 8,771,996 B2 · Marine bacterium of metabolizing 3,6-anhydro-L-galactose and use of the same

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,771,996 B2
- Document ID: US8771996B2
- Status: Granted
- Kind: B2
- Application number: 13/557,803
- Filed: 2012-07-25
- Published: 2013-04-25
- Granted: 2014-07-08
- Earliest claimed: 2011-07-26
- Assignees: Korea University Research and Business Foundation
- Applicants: Kyoung Heon Kim; In Geol Choi; Eun Ju Yun; Sae Young Lee
- Inventors: Kyoung Heon Kim; In Geol Choi; Eun Ju Yun; Sae Young Lee
- Web page: https://patentgrep.com/patent/US8771996B2

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Disclosed is a novel marine microorganism which can metabolize 3,6-anhydro-L-galactose and use of the same. The novel marine microorganism belongs to Vibrio sp. which utilizes 3,6-anhydro-L-galactose (3,6-L-AHG) as a single carbon source to convert 3,6-L-AHG to ribose and 3,6-anhydrogalactonic acid.

## Claims

### Claim 1

A marine microorganism, Vibrio sp. EJY3, that has been grown using 3,6-anhydro-L-galactose (3,6-L-AHG) as a single carbon source and is deposited under the Accession No. KCTC 11976BP.

### Claim 2 (depends on 1)

The marine microorganism, Vibrio sp. EJY3, according to claim 1, wherein the marine microorganism metabolizes 3,6-L-AHG to produce ribose and 3,6-anhydro galactonic acid.

### Claim 3 (depends on 1)

A method of culturing a novel marine microorganism, Vibrio sp. EIY3, according to claim 1, the method comprising:
culturing the marine microorganism, Vibrio sp. EIY3, using 3,6-L-AHG as a carbon source.

### Claim 4 (depends on 1)

A method of producing ribose and 3,6-anhydrogalactonic acid, comprising: producing ribose and 3,6-anhydrogalactonic acid by reacting a culture broth of the novel marine microorganism, Vibrio sp. EIY3, according to claim 1 or a crude enzyme extract thereof with 3,6-L-AHG.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the crude enzyme extract includes a water soluble protein obtained by sonicating and centrifuging the culture broth of the novel marine microorganism.

### Claim 6 (depends on 4)

The method acid according to claim 4, wherein the reaction is conducted by further adding NADH.