# US 8,771,981 B2 · Method for producing an L-amino acid

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,771,981 B2
- Document ID: US8771981B2
- Status: Granted
- Kind: B2
- Application number: 13/360,016
- Filed: 2012-01-27
- Published: 2012-08-09
- Granted: 2014-07-08
- Earliest claimed: 2009-07-29
- Assignees: Ajinomoto Co., Inc.
- Applicants: Shigeo Suzuki
- Inventors: Shigeo Suzuki
- Web page: https://patentgrep.com/patent/US8771981B2

## CPC

- C12P 13/04: Alpha- or beta- amino acids {(other amino acids C12P13/005)}
- C12N 1/20: Bacteria; Culture media therefor
- C12P 13/08: Lysine; Diaminopimelic acid; Threonine; Valine
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A method for producing an L-amino acid by preparing a processed product of a microalgae, which promotes production and accumulation of the L-amino acid by a bacterium having an ability to produce the L-amino acid, by culturing the microalgae in a medium, and processing the resulting culture at a midtemperature; culturing the bacterium in a medium containing the processed product of the microalgae to produce and accumulate the L-amino acid in culture; and collecting the L-amino acid from the culture.

## Claims

### Claim 1

A method for producing an L-amino acid, which comprises:
(a) preparing a processed product of a microalga, which promotes production and accumulation of the L-amino acid by a bacterium having an ability to produce the L-amino acid, comprising:
(i) culturing the microalga in a first medium, followed by
(ii) incubating the cultured microalga of step (i) at a mid-temperature of 40° C. to 70° C., and
(iii) performing centrifugation on the product of step (ii), resulting in a precipitate and supernatant; and
(b) culturing the bacterium in a second medium comprising the supernatant of step (iii) to produce and accumulate the L-amino acid in the second medium, wherein the supernatant comprises glucose or glycerol; and
(c) collecting the L-amino acid from the second medium.

### Claim 2 (depends on 1)

The method according to claim 1, wherein temperature is lowered the incubating of step (ii).

### Claim 3 (depends on 1)

The method according to claim 1, wherein the microalga is an alga belonging to the division Chlorophyta or Heterokontophyta.

### Claim 4 (depends on 3)

The method according to claim 3, wherein the microalga is an alga belonging to the class Chlorophyceae, Trebouxiophyceae, or Bacillariophyceae.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the microalga is an alga belonging to the class Chlorophyceae.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the bacterium belongs to the family Enterobacteriaceae or a coryneform bacterium.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the bacterium belonging to the family Enterobacteriaceae is Escherichia coli.