# US 8,771,959 B2 · Methods for the production of plants resistant to HPPD herbicides

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- Number: US 8,771,959 B2
- Document ID: US8771959B2
- Status: Granted
- Kind: B2
- Application number: 11/975,801
- Filed: 2007-10-22
- Published: 2009-01-29
- Granted: 2014-07-08
- Earliest claimed: 2000-12-07
- Assignees: Syngenta Limited
- Applicants: Christopher John Andrews; Simon Anthony James Warner; Timothy Robert Hawkes
- Inventors: Christopher John Andrews; Simon Anthony James Warner; Timothy Robert Hawkes
- Web page: https://patentgrep.com/patent/US8771959B2

## CPC

- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12N 9/0069: {acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)}
- C12N 15/8274: {for herbicide resistance}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Methods for making transgenic plants that are resistant to HPPD herbicides are presented. Polynucleotides other than those from Pseudomonas fluorescens that encode resistant HPPD enzymes are enclosed for use in the process of making transgenic plants that are tolerant to HPPD-inhibiting herbicides.

## Claims

### Claim 1

A method for selecting resistant HPPD polynucleotides which comprise a region encoding an HPPD enzyme, comprising:
(a) obtaining polynucleotides that encode HPPD enzymes capable of forming a complex with an HPPD herbicide;
(b) measuring the dissociation constant or dissociation rate constant of the complex between the encoded HPPD enzymes and the HPPD herbicide; and
(c) selecting the HPPD polynucleotides that encode HPPD enzymes that, in water at pH 7.0 and 25° C., have a dissociation constant of said complex (Kd) within the range of 1.0 to 30 nM or a dissociation rate constant within the range of 4×10−5 to 2×10−3s−1.

### Claim 2 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 2-(Nitro-4-methanesulphonylbenzoyl)-cyclohexane-1,3-dione.

### Claim 3 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 3-[[2-methyl-6-(trifluoromethyl)-3-pyridinyl]carbonyl]-bicyclo[3.2.1]-octane-2,4-dione.

### Claim 4 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 3-[[2-(ethoxymethyl)-6-(trifluoromethyl)-3-pyridinyl]carbonyl]-bicyclo[3.2.1]-octane-2,4-dione.

### Claim 5 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 1-[2-(methanesulfonyl)-4-(trifluoromethyl)phenyl]-cyclopropyl-2-cyano-propane-1,3-dione.

### Claim 6 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 2-[2-nitro-4-chlorobenzoyl]-4,4,6,6-tetramethylcyclohexane-1,3,5-trione.

### Claim 7 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 2-[2-methyl-4-cyanobenzoyl]-4,4,6,6-tetramethylcyclohexane-1,3,5-trione.

### Claim 8 (depends on 1)

The method of claim 1, wherein the HPPD herbicide is 3-[[2-methyl-6-(trifluoromethyl)-3-pyridinyl]carbonyl]-4,4,6,6,-tetramethylcyclohexane-1,3,5-trione.