# US 8,771,931 B2 · Perfusion culture method and perfusion culture device for organ or tissue

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- Number: US 8,771,931 B2
- Document ID: US8771931B2
- Status: Granted
- Kind: B2
- Application number: 13/574,855
- Filed: 2011-01-25
- Published: 2013-01-17
- Granted: 2014-07-08
- Earliest claimed: 2010-01-29
- Assignees: Organ Technologies, Inc.
- Applicants: Takashi Tsuji; Kazuhisa Nakao
- Inventors: Takashi Tsuji; Kazuhisa Nakao
- Web page: https://patentgrep.com/patent/US8771931B2

## CPC

- A01N 1/143: for organ perfusion
- C12M 21/08: {for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00)}
- C12M 29/10: {Perfusion}
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

Disclosed are a perfusion culture method and a perfusion culture device whereby a perfusate can be delivered to all parts of an organ or tissue so that the organ or tissue can be preserved while sufficiently maintaining the function thereof. Specifically disclosed is a method for perfusion culture of an organ or tissue removed from a mammal, said organ or tissue having been removed together with a second organ or tissue connected to the aforesaid organ or tissue in vivo, which comprises a step for fixing said second organ or tissue so as to hang the aforesaid organ or tissue and a step for perfusing blood vessels in the aforesaid organ or tissue with a perfusate.

## Claims

### Claim 1

A method for perfusion culture of an organ or tissue removed from a mammal, wherein said organ or tissue is removed together with a second organ or tissue which is connected to the organ or tissue in vivo, wherein the second tissue or organ is not a blood vessel, comprising: hanging the organ or tissue by fixing the second organ or tissue, and perfusing the blood vessels in the organ or tissue with a perfusate.

### Claim 2 (depends on 1)

The perfusion culture method according to claim 1, wherein in the said perfusion step, at least a part of the organ or tissue is immersed in a liquid.

### Claim 3 (depends on 2)

The perfusion culture method according to claim 2, wherein the organ or tissue is a liver, and the second organ or tissue is a diaphragm.

### Claim 4 (depends on 2)

The perfusion culture method according to claim 2, wherein the organ or tissue is a liver, and the second organ or tissue a diaphragm and a rib.

### Claim 5 (depends on 2)

The perfusion culture method according to claim 2, wherein the organ or tissue is a kidney, and the second organ or tissue is adipose tissue surrounding the kidney, wherein the adipose tissue is connected to the kidney.

### Claim 6 (depends on 1)

The perfusion culture method according to claim 1, wherein the organ or tissue is a liver, and the second organ or tissue is a diaphragm.

### Claim 7 (depends on 1)

The perfusion culture method according to claim 1, wherein the organ or tissue is a liver, and the second organ or tissue is a diaphragm and a rib.

### Claim 8 (depends on 1)

The perfusion culture method according to claim 1, wherein the organ or tissue is a kidney, and the second organ or tissue is adipose tissue surrounding the kidney, wherein the adipose tissue is connected to the kidney.