# US 8,771,767 B2 · Microbiological reprocessing of degumming residue formed during biodiesel production

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- Number: US 8,771,767 B2
- Document ID: US8771767B2
- Status: Granted
- Kind: B2
- Application number: 12/989,101
- Filed: 2009-04-22
- Published: 2011-04-28
- Granted: 2014-07-08
- Earliest claimed: 2008-04-23
- Assignees: Skotan Spolka Akcyjna
- Applicants: Franciszek Baszczok; Waldemar Rymowicz
- Inventors: Franciszek Baszczok; Waldemar Rymowicz
- Web page: https://patentgrep.com/patent/US8771767B2

## CPC

- A23J 1/18: from yeasts
- A23K 10/18: of live microorganisms
- Y02E 50/10: Biofuels, e.g. bio-diesel
- Y02P 30/20: using bio-feedstock
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23K: FODDER
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

An industrial method of reprocessing degumming residue from the initial purification of natural fats as well as a feed additive, which may be produced using the said method.

## Claims

### Claim 1

A process of producing biomass from degumming residue, comprising culturing a yeast culture of the species Yarrowia lipolytica on a medium comprising an aqueous solution containing, as a carbon source, from 20.0 to 70.0 g of degumming residue per liter of the aqueous solution, at a temperature below 34° C., with medium oxygenation in excess of 20% of O2 saturation, maintained at a pH of 2.5 to 7.5, essentially until the exhaustion of the carbon source contained in the medium, to obtain a cultured broth containing biomass, so as to thereby produce biomass from degumming residue;
wherein degumming residue comprises phosphoric acid residues and contains water, 0-10% fats, 0-10% protein, 0-5% ash and 0-1% glycerol.

### Claim 2 (depends on 1)

The process of claim 1, wherein the medium additionally contains at least one component selected from the group consisting of ammonium sulphate, potassium phosphate, magnesium sulphate, urea, thiamine, sodium hydroxide, yeast extract, corn mash, Chitosan and Acepol, at a concentration of 0.5 to 15 g/L medium.

### Claim 3 (depends on 1)

The process of claim 1, wherein the yeast culture is maintained at a pH from about 3.4 to about 3.6, and exhaustion of the carbon source is signaled by a pH increase to 4.5.

### Claim 4 (depends on 1)

The process of claim 1, further comprising spray-drying the biomass, at a temperature of about 200° C. at an input and about 90° C. at a tunnel output.

### Claim 5 (depends on 1)

The claim 1, wherein the yeast culture is maintained in a volume of about 1000 liters.

### Claim 6 (depends on 1)

The process of claim 1, wherein 15 to 35 g/L of yeast dry mass is obtained from the culture broth.

### Claim 7 (depends on 1)

The process of claim 1, wherein the biomass is produced at a rate of 1.5 to 3.0 g/L·h.

### Claim 8 (depends on 1)

The process of claim 1, wherein protein content in the biomass is from 30 to 50% by dry mass.

### Claim 9 (depends on 1)

The process of claim 1, wherein the yeast is of the Yarrowia lipolytica SKOTAN strain isolated and deposited in the IBPRS under the accession number KKP 2018 p.

### Claim 10 (depends on 1)

The process of claim 1, wherein the yeast culture is maintained in a periodically repeated way by replacing a portion of the cultured broth with a fresh portion of the medium after each exhaustion of the carbon source.

### Claim 11 (depends on 1)

The process of claim 1, wherein the yeast culture is at a temperature of about 28° C. to about 31° C.

### Claim 12 (depends on 1)

The process of claim 1, wherein the yeast culture is at a pH of 3.5±0.1.