# US 8,771,766 B2 · Genetic cluster of strains of Streptococcus thermophilus having unique rheological properties for dairy fermentation

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- Number: US 8,771,766 B2
- Document ID: US8771766B2
- Status: Granted
- Kind: B2
- Application number: 13/139,148
- Filed: 2009-12-14
- Published: 2011-09-29
- Granted: 2014-07-08
- Earliest claimed: 2008-12-12
- Assignees: Dupont Nutrition Biosciences Aps
- Applicants: Elise Manoury; Philippe Horvath; Christophe Fremaux; Pascal Fourcassie
- Inventors: Elise Manoury; Philippe Horvath; Christophe Fremaux; Pascal Fourcassie
- Web page: https://patentgrep.com/patent/US8771766B2

## CPC

- A23C 9/1238: {using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus}
- A23C 19/00: Cheese; Cheese preparations; Making thereof (cheese substitutes A23C20/00; preservation of cheese or cheese preparations A23B11/60)
- C12N 1/205: Bacterial isolates
- A23V 2400/249: Thermophilus
- C12R 2001/46: Streptococcus {; Enterococcus; Lactococcus}
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The invention relates to a genetic cluster of strains of S. thermophilus, wherein the milk fermented with said strains is highly viscous and weakly ropy. The invention also relates to a genetic cluster of strains of S. thermophilus, wherein the genome of said strains comprises a CRISPR locus having the nucleotide sequence of SEQ ID No 1.

## Claims

### Claim 1

A strain of Streptococcus thermophilus, wherein the genome of said strain comprises a CRISPR locus having the nucleotide sequence of SEQ ID No1 or a derivative thereof having at least 90% identity with SEQ ID No1, wherein when said strain ferments milk, the fermented milk has a viscosity greater than about 55 Pa·s when measured with Test A and a Caber Relative Break-up Time of less than about 0.015 seconds when measured with Test B.

### Claim 2

A strain of Streptococcus thermophilus, wherein the genome of said strain comprises a CRISPR locus comprising a nucleotide sequence selected from the group consisting of SEQ ID No2, SEQ ID No3, SEQ ID No4, SEQ ID No5, SEQ ID No6, SEQ ID No7, SEQ ID No8, SEQ ID No9, SEQ ID No10, SEQ ID No11, and SEQ ID No12 and a derivative nucleotide sequence having at least 90% identity with SEQ ID No2, SEQ ID No3, SEQ ID No4, SEQ ID No5, SEQ ID No6, SEQ ID No7, SEQ ID No8, SEQ ID No9, SEQ ID No10, SEQ ID No11 or SEQ ID No12 and wherein when said strain ferments milk, the fermented milk has a viscosity greater than about 55 Pa·s when measured with Test A and a Caber Relative Break-up Time of less than about 0.015 seconds when measured with Test B.

### Claim 3 (depends on 1, 2)

The strain according to any one of claim 1 or 2, wherein said strain is the strain of Streptococcus thermophilus deposited under the Budapest Treaty on 7th of Oct. 2008 in the name of Danisco Deutschland GmbH at the Deutsche Sammlung von Mikroorganismen und Zellkulturen under number DSM 21892 or a mutant thereof.

### Claim 4

A strain of Streptococcus thermophilus deposited under the Budapest Treaty on 7th of Oct. 2008 in the name of Danisco Deutschland GmbH at the Deutsche Sammlung von Mikroorganismen und Zellkulturen under number DSM 21892.

### Claim 5 (depends on 2)

A dairy product comprising a strain as defined in claim 2.

### Claim 6 (depends on 1)

The strain according to claim 1, wherein said derivative has at least 95% of identity with SEQ ID No1.

### Claim 7 (depends on 1)

The strain according to claim 1, wherein said derivative has at least 98% of identity with SEQ ID No1.

### Claim 8 (depends on 1)

The strain according to claim 1, wherein said derivative has at least 99% of identity with SEQ ID No1.

### Claim 9 (depends on 2)

The strain according to claim 2, wherein said derivative has at least 94% of identity with SEQ ID No2, SEQ ID No3, SEQ ID No4, SEQ ID No5, SEQ ID No6, SEQ ID No7SEQ ID No8, SEQ ID No9, SEQ ID No10, SEQ ID No11 or SEQ ID No12.

### Claim 10 (depends on 2)

The strain according to claim 2, wherein said derivative has at least 96% of identity with SEQ ID No2, SEQ ID No3, SEQ ID No4, SEQ ID No5, SEQ ID No6, SEQ ID No7, SEQ ID No8, SEQ ID No9, SEQ ID No10, SEQ ID No11 or SEQ ID No12.

### Claim 11 (depends on 2)

The strain according to claim 2, wherein said derivative has at least 99% of identity with SEQ ID No2, SEQ ID No3, SEQ ID No4, SEQ ID No5, SEQ ID No6, SEQ ID No7, SEQ ID No8, SEQ ID No9, SEQ ID No10, SEQ ID No11 or SEQ ID No12.