# US 8,771,758 B2 · Use of Tiliacora triandra in cosmetics and compositions thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,771,758 B2 - Document ID: US8771758B2 - Status: Granted - Kind: B2 - Application number: 12/827,001 - Filed: 2010-06-30 - Published: 2012-01-05 - Granted: 2014-07-08 - Earliest claimed: 2010-06-30 - Assignees: Avon Products, Inc. - Applicants: Dmitri S. Ptchelintsev - Inventors: Dmitri S. Ptchelintsev - Web page: https://patentgrep.com/patent/US8771758B2 ## CPC - A61K 8/9761: Cupressaceae [Cypress family], e.g. juniper or cypress - A61K 8/9789: Magnoliopsida [dicotyledons] - A61K 8/9794: Liliopsida [monocotyledons] - A61Q 19/08: Anti-ageing preparations - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS ## Abstract The present disclosure relates to compositions and methods for treating, preventing and improving the condition and aesthetic appearance of skin, particularly, treating, preventing, ameliorating, reducing and/or eliminating fine lines and/or wrinkles of skin, where the compositions include natural plant constituents which increase expression levels genes, collagen replacement and retention, and cell proliferation of epidermis and dermis associated with the dermatological signs of aging. The compositions of the invention are topically applied to the skin, or are delivered by directed means to a site in need thereof, once daily in an amount effective in improving the condition and aesthetic appearance of skin. ## Claims ### Claim 1 A method of improving the aesthetic appearance of aging skin by lessening one or more of facial lines, wrinkles, or sagging skin comprising topically applying to skin affected by facial lines, wrinkles, or sagging, a composition comprising: (a) a Tiliacora triandra Diels plant extract capable of inhibiting collagenase activity in human fibroblasts and present in an amount from about 0.01 wt % to about 10 wt % based on the total weight of the composition; and (b) a cosmetically or physiologically acceptable vehicle, the composition being applied in an amount and for a time effective to lessen said facial lines, wrinkles, or sagging skin; wherein the plant extract is obtained by extraction with a solvent mixture comprising from about 60% by volume to about 90% by volume ethanol and from about 10% by volume and to about 40% by volume water. ### Claim 2 (depends on 1) The method according to claim 1, wherein the composition is topically applied at least once daily for at least one week. ### Claim 3 (depends on 2) The method of claim 2, wherein the plant extract is dried. ### Claim 4 (depends on 2) The method of claim 2, wherein the plant extract is from a vine of Tiliacora triandra Diels. ### Claim 5 (depends on 4) The method of claim 4, wherein the plant extract is obtained by extraction with the solvent mixture for about 1 to about 8 hours at a temperature of about 30° to 90° C. ### Claim 6 (depends on 5) The method of claim 5, wherein the plant extract is dried. ### Claim 7 (depends on 1) The method of claim 1, wherein the composition is essentially free of iodine. ### Claim 8 (depends on 1) The method of claim 1, wherein the composition is essentially free of hydrogen peroxide. ### Claim 9 (depends on 1) The method according to claim 1, wherein the composition is applied directly to a wrinkle. ### Claim 10 A method of improving the aesthetic appearance of aging skin by improving skin thickness comprising topically applying to skin in need of improvement in thickness, a composition comprising: (a) a Tiliacora triandra Diels plant extract capable of inhibiting collagenase activity in human fibroblasts, and present in an amount from about 0.01 wt % to about 10 wt % based on the total weight of the composition; and (b) a cosmetically or physiologically acceptable vehicle, the composition being applied in an amount and for a time effective to improve said skin thickness; wherein the plant extract is obtained by extraction with a solvent mixture comprising from about 60% by volume to about 90% by volume ethanol and from about 10% by volume to about 40% by volume water. ### Claim 11 (depends on 10) The method according to claim 10, wherein the composition is topically applied at least once daily for at least one week. ### Claim 12 (depends on 11) The method of claim 11, wherein the extract is dried. ### Claim 13 (depends on 11) The method of claim 11, wherein the plant extract is from a vine of Tiliacora triandra Diels. ### Claim 14 (depends on 10) The method of claim 10, wherein the plant extract is from a vine of Tiliacora triandra Diels. ### Claim 15 (depends on 14) The method of claim 14, wherein the plant extract is obtained by extraction with the solvent mixture for about 1 to about 8 hours at a temperature of about 30° to 90° C. ### Claim 16 (depends on 15) The method of claim 15, wherein the plant extract is dried. ### Claim 17 (depends on 10) The method of claim 10, wherein the composition is essentially free of iodine. ### Claim 18 (depends on 10) The method of claim 10, wherein the composition is essentially free of hydrogen peroxide. ### Claim 19 A method for improving the barrier function of the skin, comprising topically applying to skin in need of improvement in the barrier function a composition comprising: (a) a Tiliacora triandra Diels plant extract capable of inhibiting collagenase activity in human fibroblasts and present in an amount from about 0.01 wt % to about 10 wt % based on the total weight of the composition; and (b) a cosmetically or physiologically acceptable vehicle, the composition being applied in an amount and for a time effective to improve barrier function; wherein the plant extract is obtained by extraction with a solvent mixture comprising from about 60% by volume to about 90% by volume ethanol and from about 10% by volume to about 40% by volume water. ### Claim 20 (depends on 19) The method according to claim 19, wherein the composition is topically applied at least once daily for at least one week. ### Claim 21 (depends on 20) The method according to claim 20, wherein the plant extract is derived from a vine of Tiliacora triandra Diels.