# US 8,771,708 B2 · Biologically-active radiolabeled Cry1Fa and receptor binding assay methods

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- Number: US 8,771,708 B2
- Document ID: US8771708B2
- Status: Granted
- Kind: B2
- Application number: 13/327,842
- Filed: 2011-12-16
- Published: 2012-06-21
- Granted: 2014-07-08
- Earliest claimed: 2010-12-16
- Assignees: Dow AgroSciences LLC
- Applicants: Steven L. Evans; Jianquan Li; Joel J. Sheets
- Inventors: Steven L. Evans; Jianquan Li; Joel J. Sheets
- Web page: https://patentgrep.com/patent/US8771708B2

## CPC

- C07K 14/325: Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.

## Claims

### Claim 1

A Cry1Fa holotoxin labeled with radioiodinated fluorescein-5-maleimide at position C205, said radiolabeled holotoxin retaining the ability to selectively bind to receptors in brush border membrane vesicles prepared from midguts of Spodoptera exigua and Spodoptera frugiperda.

### Claim 2 (depends on 1)

The Cry1Fa holotoxin of claim 1, said radiolabeled Cry1Fa holotoxin having insecticidal activity.