# US 8,771,704 B2 · Dengue vaccine, pharmaceutical composition comprising the same, nucleotide sequence and antibody composition

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,771,704 B2
- Document ID: US8771704B2
- Status: Granted
- Kind: B2
- Application number: 13/230,209
- Filed: 2011-09-12
- Published: 2012-03-22
- Granted: 2014-07-08
- Earliest claimed: 2010-09-13
- Assignees: National Cheng Kung University
- Applicants: Yee-Shin Lin
- Inventors: Yee-Shin Lin
- Web page: https://patentgrep.com/patent/US8771704B2

## CPC

- A61K 39/12: Viral antigens
- A61P 31/12: Antivirals
- A61P 31/14: for RNA viruses
- C07K 14/005: from viruses
- C07K 16/116: Togaviridae (F); Matonaviridae (F); Flaviviridae (F)
- A61K 2039/505: {comprising antibodies}
- C12N 2770/24122: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2770/24134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention relates to a dengue vaccine, a pharmaceutical composition comprising the same, a nucleotide sequence, and an antibody composition. The dengue vaccine of the present invention includes chimeric nonstructural protein 1, which comprises N-terminus of DV NS1 from amino-acid residues 1 to 270 and C-terminus of JEV NS1 from amino-acid residues 271 to 352 (designated DJ NS1). Therefore, the dengue vaccine without autoimmunity according to the present invention is able to avoid cross-reactions with endothelial cells and platelets, and is able to shorten the bleeding time.

## Claims

### Claim 1

A composition comprising a chimeric nonstructural protein DJ NS1, wherein the chimeric nonstructural protein DJ NS1 comprises:
a sequence having at least 95% sequence identity with the N-terminus of the dengue virus nonstructural protein 1 (DV NS1) from amino acids 1 to 270 of SEQ ID NO: 1; and
a sequence having at least 95% sequence identity with the C-terminus of Japanese encephalitis virus nonstructural protein 1 (JEV NS1) from amino acids 271 to 352 of SEQ ID NO: 1.

### Claim 2

A composition comprising a chimeric nonstructural protein DJ NS1, wherein the chimeric nonstructural protein DJ NS1 comprises an amino acid sequence having at least 95% sequence identity with SEQ ID NO: 1.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the amino acid sequence of the chimeric nonstructural protein DJ NS1 is SEQ ID NO: 1.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the N-terminus of the dengue virus nonstructural protein 1 (DV NS1) from amino acids 1 to 270 is derived from dengue virus protein.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the C-terminus of the Japanese encephalitis virus nonstructural protein 1 (JEV NS1) from amino acids 271 to 352 is derived from Japanese encephalitis virus protein.