# US 8,771,675 B2 · Probiotic strains for pets

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- Number: US 8,771,675 B2
- Document ID: US8771675B2
- Status: Granted
- Kind: B2
- Application number: 12/053,037
- Filed: 2008-03-21
- Published: 2008-07-17
- Granted: 2014-07-08
- Earliest claimed: 2000-05-25
- Assignees: Nestec S.A.
- Applicants: Ralf Zink; Roberto Reniero; Florence Rochat; Christoph Cavadini; Thierry Von Der Weid; Eduardo Schiffrin; Jalil Benyacoub; Virginie Rousseau; Pablo Perez; Ruth Knorr
- Inventors: Ralf Zink; Roberto Reniero; Florence Rochat; Christoph Cavadini; Thierry Von Der Weid; Eduardo Schiffrin; Jalil Benyacoub; Virginie Rousseau; Pablo Perez; Ruth Knorr
- Web page: https://patentgrep.com/patent/US8771675B2

## CPC

- C12N 1/205: Bacterial isolates
- A23K 10/18: of live microorganisms
- A23K 50/42: Dry feed
- A23K 50/48: Moist feed
- A61K 35/744: Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K 35/745: Bifidobacteria
- A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis
- A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system
- A61P 17/00: Drugs for dermatological disorders
- A61P 31/04: Antibacterial agents
- A61P 31/12: Antivirals
- A61P 37/02: Immunomodulators
- A61P 37/04: Immunostimulants
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C12R 2001/225: Lactobacillus
- C12R 2001/23: Lactobacillus acidophilus
- C12R 2001/46: Streptococcus {; Enterococcus; Lactococcus}
- Y10S 426/805: Pet food for dog, cat, bird, or fish
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23K: FODDER
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

The present invention provides novel strains of probiotics for use in the gastrointestinal tract of a pet. The probiotics of the present invention are capable of fermenting starch to produce lactic acid and/or hydrogen peroxide anti-pathogenic metabolites. The present invention also provides a novel method for identifying the effectiveness of a pet probiotic in vitro. The method simulates the introduction of a probiotic candidate in the gastrointestinal tract of a pet and measures the anti-pathogenic effectiveness of the probiotic.

## Claims

### Claim 1

A method for producing a pet food, the method comprising:
providing a probiotic;
exposing the probiotic to a gastric juice;
providing a model of a pet small intestine;
introducing the probiotic and a pathogen into the model;
measuring the amount of an anti-pathogenic metabolite produced by the probiotic; and
adding the provided probiotic to an ingestible composition for pets in an amount sufficient to produce an effective anti-pathogenic metabolite,
wherein the probiotic is a bacterial strain selected from the group consisting of Lactobacillus acidophilus NCC 2766 (CNCM I-3848), Lactobacillus acidophilus NCC 2775 (CNCM I-3851), Lactobacillus acidophilus NCC 2628 (CNCM I-2453), Lactobacillus johnsonii NCC 2777 (CNCM I-3852), Lactobacillus johnsonii NCC 2767 (CNCM I-3849), Lactobacillus johnsonii NCC 2774 (CNCM I-3850), and Lactobacillus salivarius NCC 2586 (CNCM I-3847).

### Claim 2 (depends on 1)

The method of claim 1, wherein the pet is a canine.

### Claim 3 (depends on 1)

The method of claim 1, wherein the pet food is dog food.

### Claim 4 (depends on 1)

The method of claim 1, wherein the provided probiotic produces an anti-pathogenic metabolite that lowers the pH of the gastrointestinal tract locally to a range of from about 4.5 to about 5.5.

### Claim 5 (depends on 1)

The method of claim 1, wherein the provided probiotic produces an anti-pathogenic metabolite that inhibits the growth of a pathogen present in the gastrointestinal tract, the pathogen selected from the group consisting of enterotoxigenic Escherichia coli, Escherichia coli, Salmonella typhimurium, Shigella dysenteriae and combinations thereof.

### Claim 6 (depends on 1)

The method of claim 1, wherein the pathogen introduced into the model is selected from the group consisting of enterotoxigenic Escherichia coli, Escherichia coli, Salmonella typhimurium, Shigella dyvsenteriae and combinations thereof.

### Claim 7 (depends on 1)

The method of claim 1, wherein the anti-pathogenic metabolite produced by the probiotic is selected from the group consisting of lactic acid, acetic acid, citric acid, pyruvic acid, hydrogen peroxide, and combinations thereof.

### Claim 8 (depends on 1)

The method of claim 1, wherein the provided probiotic produces lactic acid in an amount of from about 10 mM to about 90 mM.

### Claim 9 (depends on 1)

The method of claim 1, wherein the provided probiotic produces hydrogen peroxide in an amount of from about 0.1 mM to about 4.5 mM.

### Claim 10 (depends on 1)

The method of claim 1, wherein the provided probiotic is capable of surviving in a pH 2.6 environment for at least 10 minutes.

### Claim 11 (depends on 1)

The method of claim 1, wherein the provided probiotic is capable of surviving in a pH 3.4 environment for at least 60 minutes.

### Claim 12 (depends on 1)

The method of claim 1, wherein the provided probiotic is obtained from a fecal sample of a dog or a cat.