# US 8,771,667 B2 · Tctex-1 regulatory sequence as stem cell marker

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- Number: US 8,771,667 B2
- Document ID: US8771667B2
- Status: Granted
- Kind: B2
- Application number: 13/496,974
- Filed: 2010-09-20
- Published: 2012-07-19
- Granted: 2014-07-08
- Earliest claimed: 2009-09-18
- Assignees: Cornell University
- Applicants: Ching-Hwa Sung; Jen-Zen Chuang
- Inventors: Ching-Hwa Sung; Jen-Zen Chuang
- Web page: https://patentgrep.com/patent/US8771667B2

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- A61P 25/00: Drugs for disorders of the nervous system
- C12Q 2600/106: Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The disclosure relates to an isolated regulatory sequence for Tctex-1 that is transcriptionally active in adult neural progenitor and stem cells, including Type 1, Type 2 and Type 3 progenitors, as well as during development. The disclosure also relates to a method for selectively expressing a genetic sequence in neural progenitor cells. The disclosure of inserting and expressing a specific sequence in these cells allows marking, identification, sorting, tracking, and manipulating neural progenitor and stem cells.

## Claims

### Claim 1

A vector comprising a nucleic acid encoding a Tctex-1 promoter operably linked to a heterologous nucleic acid, said promoter comprising the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2.

### Claim 2 (depends on 1)

The vector of claim 1, wherein said nucleic acid of interest encodes a marker gene.

### Claim 3 (depends on 1)

The vector of claim 1, wherein said nucleic acid of interest encodes an effector nucleic acid.

### Claim 4 (depends on 1)

The vector of claim 1, wherein said nucleic acid of interest encodes a protein of interest.

### Claim 5 (depends on 1, 2, 3, 4)

A host cell comprising a vector of any one of claims 1-4.

### Claim 6 (depends on 1, 2, 3, 4)

A pharmaceutical composition comprising a vector of any one of claims 1-4.

### Claim 7 (depends on 5)

A pharmaceutical composition comprising the host cell of claim 5.

### Claim 8 (depends on 1, 2, 3, 4)

A kit comprising a vector of any one of claims 1-4.