# US 8,771,647 B2 · Human photoaged skin model > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,771,647 B2 - Document ID: US8771647B2 - Status: Granted - Kind: B2 - Application number: 11/766,522 - Filed: 2007-06-21 - Published: 2008-01-10 - Granted: 2014-07-08 - Earliest claimed: 2005-12-20 - Assignees: Kao Corporation - Applicants: Akira Hachiya; Tsutomu Fujimura - Inventors: Akira Hachiya; Tsutomu Fujimura - Web page: https://patentgrep.com/patent/US8771647B2 ## CPC - A01K 67/0271: Chimeric vertebrates, e.g. comprising exogenous cells - A01K 2227/105: Murine - A01K 2267/035: Animal model for multifactorial diseases - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS ## Abstract The present invention provides a human photoaged skin model and an animal model, and a method of making the same, for evaluating cosmetics and similar products in terms of their anti-aging or rejuvenating effect. ## Claims ### Claim 1 A method for producing a human photoaged skin model, comprising: irradiating a skin area of an immunodeficient mouse; and discontinuing irradiation when the skin area is in a condition suitable for use as a human photoaged skin model; wherein: the skin area comprises human skin transplanted to the immunodeficient mouse; irradiating the skin area comprises irradiating for a period of from six to eight consecutive weeks, during which: the skin area is irradiated with 40 to 50 mJ/cm2 of UV-B radiation during a first week of the period; the skin area is irradiated with 50 to 100 mJ/cm2 of UV-B radiation during a second week of the period; the skin area is irradiated with 60 to 100 mJ/cm2 of UV-B radiation during a third week, a fourth week, a fifth week, and a sixth week of the period; and the skin area is optionally irradiated with 60 to 100 mJ/cm2 of UV-B radiation during an optional seventh week and an optional eighth week of the period; irradiating the skin area comprises irradiating with a total dose of UV-B radiation of from approximately 1.65 J/cm2 to approximately 2.85 J/cm2; and the skin area is in the condition suitable for use as a human photoaged skin model when the skin area has at least one condition selected from the group consisting of wrinkles, sagginess, age spots, freckles, lowered elasticity, increased darkening, increased yellowing, and reduced keratinous layer water content. ### Claim 2 (depends on 1) The method according to claim 1, wherein the immunodeficient mouse is a SCID mouse. ### Claim 3 (depends on 2) The method according to claim 2, wherein the SCID mouse is BALB cA-nu/SCID or B-17/Icr-SCID. ### Claim 4 A method for producing a human photoaged skin model, comprising: irradiating a skin area of an immunodeficient mouse; and discontinuing irradiation when the skin area is in a condition suitable for use as a human photoaged skin model; wherein: the skin area comprises human skin transplanted to the immunodeficient mouse; irradiating the skin area comprises irradiating for a period of from six to eight consecutive weeks, during which: the skin area is irradiated with 80 to 90 mJ/cm2 of UV-B radiation during a first week of the period; the skin area is irradiated with 90 to 100 mJ/cm2 of UV-B radiation during a second week of the period; the skin area is irradiated with 100 mJ/cm2 of UV-B radiation during a third week, a fourth week, a fifth week, and a sixth week of the period; and the skin area is optionally irradiated with 100 mJ/cm2 of UV-B radiation during an optional seventh week and an optional eighth week of the period; irradiating the skin area comprises irradiating with a total dose of UV-B radiation of from approximately 1.65 J/cm2 to approximately 2.85 J/cm2; and the skin area is in the condition suitable for use as a human photoaged skin model when the skin area has at least one condition selected from the group consisting of wrinkles, sagginess, age spots, freckles, lowered elasticity, increased darkening, increased yellowing, and reduced keratinous layer water content. ### Claim 5 (depends on 1) The method according to claim 1, wherein irradiating the skin area comprises performing continuous irradiation. ### Claim 6 (depends on 1) The method according to claim 1, wherein irradiating the skin area comprises performing pulsed irradiation. ### Claim 7 (depends on 1) The method according to claim 1, wherein irradiating the skin area comprises irradiating five times per week. ### Claim 8 (depends on 1) The method according to claim 1, wherein the total dose of UV-B radiation is approximately 1.65 J/cm2. ### Claim 9 (depends on 1) The method according to claim 1, wherein the total dose of UV-B radiation is approximately 2.85 J/cm2. ### Claim 10 (depends on 1) An animal model which bears human photoaged skin and which is produced by the method according to claim 1. ### Claim 11 (depends on 8) The method according to claim 8, wherein the human skin is abdominal skin. ### Claim 12 (depends on 9) The method according to claim 9, wherein the human skin is foreskin. ### Claim 13 (depends on 1) The method according to claim 1, comprising allowing the skin area to rest for at least 3 weeks after irradiating the skin area. ### Claim 14 (depends on 13) The method according to claim 13, comprising allowing the skin area to rest for 3 to 4 weeks after irradiating the skin area. ### Claim 15 (depends on 4) The method according to claim 4, wherein the immunodeficient mouse is a SCID mouse. ### Claim 16 (depends on 15) The method according to claim 15, wherein the SCID mouse is BALB cA-nu/SCID or B-17/Icr-SCID. ### Claim 17 (depends on 4) The method according to claim 4, wherein irradiating the skin area comprises performing continuous irradiation. ### Claim 18 (depends on 4) The method according to claim 4, wherein irradiating the skin area comprises performing pulsed irradiation. ### Claim 19 (depends on 4) The method according to claim 4, wherein irradiating the skin area comprises irradiating five times per week. ### Claim 20 (depends on 4) The method according to claim 4, wherein the total dose of UV-B radiation is approximately 1.65 J/cm2. ### Claim 21 (depends on 4) The method according to claim 4, wherein the total dose of UV-B radiation is approximately 2.85 J/cm2. ### Claim 22 (depends on 4) An animal model which bears human photoaged skin and which is produced by the method according to claim 4. ### Claim 23 (depends on 20) The method according to claim 20, wherein the human skin is abdominal skin. ### Claim 24 (depends on 21) The method according to claim 21, wherein the human skin is foreskin. ### Claim 25 (depends on 4) The method according to claim 4, comprising allowing the skin area to rest for at least 3 weeks after irradiating the skin area. ### Claim 26 (depends on 25) The method according to claim 25, comprising allowing the skin area to rest for 3 to 4 weeks after irradiating the skin area.