# US 8,771,611 B2 · System and methods of log-scale concentration gradients > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,771,611 B2 - Document ID: US8771611B2 - Status: Granted - Kind: B2 - Application number: 13/305,645 - Filed: 2011-11-28 - Published: 2013-06-27 - Granted: 2014-07-08 - Earliest claimed: 2011-11-28 - Assignees: Auburn University; Pukyong National University - Applicants: Jong Wook Hong; Se-Kwon Kim; Jae Young Yun - Inventors: Jong Wook Hong; Se-Kwon Kim; Jae Young Yun - Web page: https://patentgrep.com/patent/US8771611B2 ## CPC - C12Q 1/37: involving peptidase or proteinase - B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip} - G01N 33/48: Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) - B01L 2200/142: Preventing evaporation - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution - Y10T 436/11: Automated chemical analysis - Y10T 436/2575: Volumetric liquid transfer - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract Devices and methods use an integrated microfluidic system that has the capability of realizing a wide range of accurate dilutions in a logarithmic way through semi-direct dilution of samples inside a chip. The device for dose response analysis is able to contain a first fluid source on a microfluidic chip, wherein the first fluid source comprises a drug, a second fluid source on the microfluidic chip, a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source, and a detection area coupling with the mixing area for drug information detection using a detection system. ## Claims ### Claim 1 A device for dose response analysis comprising: a) a first fluid source on a microfluidic chip, wherein the first fluid source comprises a drug; b) a second fluid source on the microfluidic chip; c) a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source; d) a logarithmic concentration former fluidically coupling with the first fluid source and the second fluid source; and e) a detection area coupling with the mixing area for drug information detection. ### Claim 2 (depends on 1) The device of claim 1, wherein the second fluid source comprises a control for controlling a buffer solution. ### Claim 3 (depends on 1) The device of claim 1, wherein the first fluid source comprises a control for controlling an inhibitor, an enzyme, or a chemical having a therapeutical effect. ### Claim 4 (depends on 1) The device of claim 1, wherein the microfluidic chip comprises a control to mix a first amount of fluid from the first fluid source and a second amount of fluid from the second fluid source making a first drug solution with a first concentration and a second drug solution with a second concentration, wherein the first concentration and the second concentration are logarithmically related. ### Claim 5 (depends on 1) The device of claim 1, wherein the first fluid source, the second fluid source, or both comprises a control to generate a solution in a volume equal or smaller than micro-liter. ### Claim 6 (depends on 5) The device of claim 5, wherein the volume is nano-liter. ### Claim 7 (depends on 5) The device of claim 5, wherein the volume is pico-liter. ### Claim 8 (depends on 1) The device of claim 1, wherein the drug information is received from a bacterial cell, a fungus, a mammalian cell, or a combination thereof. ### Claim 9 (depends on 1) The device of claim 1, further comprises a temperature control system capable of controlling the kinetics of a reaction or providing reaction kinetics. ### Claim 10 (depends on 1) The device of claim 1 further comprises a humidity control system capable of preventing evaporation. ### Claim 11 A device for preparing a fluid sample comprising: a) a first fluid source on a microfluidic chip, wherein the first fluid source comprises a first fluid; b) a second fluid source on the microfluidic chip, wherein the second fluid source comprises a second fluid; c) a logarithmic concentration former fluidically coupling with the first fluid source and the second fluid source; and d) a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source capable of making multiple concentrations. ### Claim 12 (depends on 11) The device of claim 11, wherein the second fluid source comprises a control for controlling a buffer solution. ### Claim 13 (depends on 11) The device of claim 11, wherein the microfluidic chip comprises a control to mix a first amount of fluid from the first fluid source and a second amount of fluid from the second fluid source making a first sample solution with a first concentration and a second sample solution with a second concentration, wherein the first concentration and the second concentration are logarithmically related. ### Claim 14 (depends on 11) The device of claim 11, wherein the first fluid source, the second fluid source, or both comprises a control to generate a solution in a volume equal or smaller than micro-liter. ### Claim 15 (depends on 14) The device of claim 14, wherein the volume is nano-liter. ### Claim 16 (depends on 14) The device of claim 14, wherein the volume is pico-liter.