Glucose sensors and methods of manufacture thereof

A method comprising:

• performing periodic biasing amperometry on a sensor, the sensor comprising:
• a reference electrode;
• a counter electrode;
• a working electrode;
• the working electrode being disposed in the vicinity of the reference and counter electrode;
• an electrically conducting membrane;
• the electrically conducting membrane being in operative communication with the working electrode;
• an enzyme layer;
• the enzyme layer being in operative communication with the working electrode;
• a semi-permeable membrane;
• the semi-permeable membrane being in operative communication with the working electrode introducing a sample comprising an analyte being measured to the sensor;
• the periodic biasing amperometry comprising:
• determining a normal operating state for the sensor by biasing the working electrode for the same duration of time at intervals (twait) having the same periods of time at a number of testing potentials;
• repeating the periodic biasing for all the testing potentials;
• continuing the periodic biasing until a steady state is attained for all the testing potentials;
• conducting an internal calibration of the sensor after the analyte being measured has reached a steady state;
• the internal calibration comprising a time interval (tcal) where the periodic biasing is not applied;
• where tcal=n×twait, where n is a number of about 2 to about 10;
• measuring a periodic biasing amperometric signal difference immediately before and immediately after the time interval (tcal):
• comparing the differential with a calibration chart to obtain sensitivity factors;
• and
• applying the sensitivity factors to the sensor to correct against drifts;
• and
• performing biasing amperometry at a time interval of teq to ensure that the sensor returns to its normal operating state;
• where teq is less than twait.