# US 8,769,869 B2 · Methods of preventing flooding in manufactured seed

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- Number: US 8,769,869 B2
- Document ID: US8769869B2
- Status: Granted
- Kind: B2
- Application number: 13/233,246
- Filed: 2011-09-15
- Published: 2012-03-29
- Granted: 2014-07-08
- Earliest claimed: 2010-09-28
- Assignees: Weyerhaeuser NR Company
- Applicants: Jeffrey E. Hartle
- Inventors: Jeffrey E. Hartle
- Web page: https://patentgrep.com/patent/US8769869B2

## CPC

- C08L 5/12: {Agar or} agar-agar {, i.e. mixture of agarose and agaropectin}; Derivatives thereof
- A01H 4/006: {Encapsulated embryos for plant reproduction, e.g. artificial seeds}
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The invention provides methods of preventing flooding of a cavity within a shoot restraint of a manufactured seed by lowering the water potential of the nutritive media relative to the water potential of a plant embryo disposed within the cavity.

## Claims

### Claim 1

A manufactured seed comprising:
(a) a seed coat;
(b) a shoot restraint, wherein the shoot restraint comprises a cavity; and
(c) a nutritive media comprising agar at a concentration from about 26 g/L to about 30 g/L, whereby the concentration of agar in the nutritive media lowers the water potential of the nutritive media, wherein the water potential of the nutritive media relative to the water potential of an embryo disposed in the cavity of the shoot restraint is such that an excessive amount of water does not flow out of the nutritive media and accumulate within the cavity, thereby preventing flooding of the cavity.

### Claim 2 (depends on 1)

The manufactured seed of claim 1, wherein the concentration of agar in the nutritive media is from about 26 g/L to about 28 g/L.

### Claim 3 (depends on 1)

The manufactured seed of claim 1, wherein the concentration of agar in the nutritive media is about 26 g/L.

### Claim 4 (depends on 1)

The manufactured seed of claim 1, further comprising an adsorbent material within the cavity of the shoot restraint.

### Claim 5 (depends on 4)

The manufactured seed of claim 4, wherein the adsorbent material is charcoal.

### Claim 6 (depends on 1)

The manufactured seed of claim 1, wherein the adsorbent material is nutrient-treated charcoal.

### Claim 7 (depends on 1)

The manufactured seed of claim 1, further comprising a plant embryo disposed in the cavity of the shoot restraint.

### Claim 8

A nutritive media comprising agar at a concentration from about 26 g/L to about 30 g/L for use in a manufactured seed, wherein the manufactured seed comprises a seed coat, a shoot restraint, and a cavity within the shoot restraint, whereby the concentration of agar in the nutritive media lowers the water potential of the nutritive media, wherein the water potential of the nutritive media relative to the water potential of an embryo disposed in the cavity of the shoot restraint is such that an excessive amount of water does not flow out of the nutritive media and accumulate within the cavity, thereby preventing flooding of the cavity.

### Claim 9 (depends on 8)

The nutritive media of claim 8, wherein the concentration of agar in the nutritive media is from about 26 g/L to about 28 g/L.

### Claim 10 (depends on 8)

The nutritive media of claim 8, wherein the concentration of agar in the nutritive media is about 26 g/L.